Cystatin C, Lipoprotein(a) and Ceruloplasmin in the Thermo Scientific Konelab Test Menu

1. Cystatin C, Lipoprotein(a) and Ceruloplasmin in the Thermo Scientific Konelab Test Menu

2. Albumin Antistreptolysin O  -1-Antitrypsin Apo A1 & B Complement C3 Complement C4 C-reactive protein High Sensitive CRP HbA1c Haptoglobin IgA, G & M Microalbuminuria Myoglobin Orosomucoid Prealbumin Rheumatoid factors Transferrin Specific Protein tests on Konelab Systems • Cystatin C • Lipoprotein(a) • Ceruloplasmin

3. Creatinine, Enzymatic Creatinine, Jaffé Urea/BUN Total protein in urine Microalbuminuria Cystatin C Renal testing Source: http://training.seer.cancer.gov/module_anatomy/unit11_2_uri_components.html

4. Kidney monitoring GFR, Glomerular Filtration Rate Traditionally with Creatinine measurement NEW MARKER – CYSTATIN C Why Cystatin C together or instead of Creatinine? Specificity Creatinine is affected by age/sex/muscle mass/inflammatory process Cystatin C test is not dependent of any listed issues Sensitivity Creatinine measurement is able to detect kidney dysfunction when app. 50% of kidney mass is ‘non-working’ Cystatin C test has the sensitivity to detect already mild renal dysfunction Other highlights Elevated serum Cystatin C is a strong predictor of the risk of death and cardiovascular events in elderly persons Cystatin C - a Novel Serum Marker for GFR

5. Easy and reliable assay Ready for use liquid reagents Method: latex enhanced immunoturbidimetry On board stability for reagents 30 days Kit size 2 x 3 ml, up to 190 tests 981911 Cystatin C One level stock calibrator Ready for use liquid Automatic dilutions for calibration curve Kit size 2 x 1 ml 981912 Cystatin C Calibrator Controls Normal and elevated levels 981913 Cystatin C Control, 3 x 1 ml 981914 Cystatin C Control High, 3 x 1 ml Cystatin C on Konelab systems

6. Total Cholesterol HDL & LDL-Cholesterol Triglycerides Apolipoprotein A1 & B Lipoprotein(a) Lipid tests

7. Sterols: Cholesterol, steroid hormones etc. Fatty acids Glycerol esters, e.g. triglycerides Sphingosines and Terpenes e.g. Vitamin A, E and K Classification of Lipids

8. Synthesized in liver 10% coming from dietary intake Transported in complexes known as lipoproteins Clinical interest in HDL and LDL Cholesterol Cholesterol Phospholipid Triglyceride + Esterified Cholesterol Free Cholesterol Apolipoprotein

9. Lipoproteins • Apolipoprotein A-I/II => HDL: “good cholesterol” • Apolipoprotein B => LDL: “bad cholesterol” • Lipoprotein(a) = LDL- molecule + Apolipoprotein (a) Phospholipid Triglyceride + Esterified Cholesterol Free Cholesterol Apolipoprotein

10. A powerful predictor for development of atherosclerotic vascular disease Lipoprotein(a) is a dimer consisting of LDL molecule bound to Apo(a) via a disulphide bridge Lp(a) is a risk factor for coronary heart disease (CHD) that is independent of all other lipid parameters Lp(a) is a special tool for doctors The CHD risk predictor is high, especially when Lp(a) and LDL are elevated simultaneously => lipid lowering therapy recommended Lipoprotein(a) Lipoprotein(a) – a ’killer molecule’

11. Easy and reliable assays Ready for use liquid reagents Method: direct immunoturbidimetry Kit size 1 x 1 ml, up to 180 tests 981915 Lipoprotein(a) One level stock calibrator Ready for use liquid Automatic dilutions for calibration curve 981916 Lipoprotein(a) Calibrator, 2 x 1 ml Controls Normal and elevated levels 981917 Lipoprotein(a) Control, 3 x 1 ml 981918 Lipoprotein(a) Control High, 3 x 1 ml k Lipoprotein(a) on Konelab Systems

12. Ceruloplasmin measurement aids to diagnose copper metabolism disorders Ceruloplasmin is the major copper–containing protein in plasma When is the Ceruloplasmin level abnormal? Decreased levels disorders of copper metabolism like Menke’s disease and Wilson’s disease Elevated levels During pregnancy With lymphoma In acute and chronic infections (acute phase protein) Rheumatoid arthritis Ceruloplasmin

13. Ready for use liquid reagents Method: direct immunoturbidimetry On board stability for reagents 30 days Kit size 2 x 3 ml, up to 190 tests 981919 Ceruloplasmin SpeciCal for calibration Ready for use liquid Automatic dilutions for calibration curve 980997 SpeciCal, 5 x 1 ml Controls 981250 SpeciTrol, 5 x 1 ml 981799 SpeciTrol High, 5 x 1 ml Ceruloplasmin on Konelab Systems

14. Albumin ACP Tot & Dir Bil ALP Bicarbonate ALT/GPT Calcium AST/GOT Cholesterol Amylase HDL&LDL Chol CHE Creatinine Dibucaine Glucose CK Iron CK-MB Magnesium Gamma-GT Phosphorus -HBDH Total Protein LDH Triglycerides Lipase U/CSF Protein Urea ELECTROLYTES Uric Acid Na, K, Cl Ca, pH Konelab Menu – over 80 carefully validated methods ENZYMES SUBSTRATES

15. Konelab Menu – over 80 carefully validated methods Albumin Antistreptolysin O  -1-Antitrypsin Apo A1 & B Complement C3 Complement C4 C-reactive protein High Sensitive CRP HbA1c Haptoglobin IgA, G & M Microalbuminuria Myoglobin Orosomucoid Prealbumin Rheumatoid factors Transferrin sTfR Cystatin C Lipoprotein(a) Ceruloplasmin Amphetamine Barbiturates Benzodiazepines Cannabinoids Cocaine Metabolite Opiates Cotinine Ethyl Alcohol Methadone Propoxyphene • Carbamazepine • Phenobarbital • Phenytoin • Theophylline • Valproic Acid • Lithium TDM DoA (DAT) SPECIFIC PROTEINS

16. Konelab System Reagents • Proven, continously expanding test menu • Delivered in optimal package sizes to serve different sized laboratories • Ready for use liquid reagents for ease of use