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Electrospinning functionalized nanofibre membrane for anti-bacterial nanofiltration systems

Electrospinning functionalized nanofibre membrane for anti-bacterial nanofiltration systems. Conor Keevey (AOS) Garrett Nekic (AOS) Darryl Chai (HCI) Sit Han Zhe (HCI). Background.

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Electrospinning functionalized nanofibre membrane for anti-bacterial nanofiltration systems

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  1. Electrospinning functionalized nanofibre membrane for anti-bacterial nanofiltration systems ConorKeevey (AOS) Garrett Nekic (AOS) Darryl Chai (HCI) Sit Han Zhe (HCI)

  2. Background • Electrospinning is a simple and versatile process that is used to create nanofibre membranes for a variety of applications. • Scientists have implemented various chemicals into the polymer solution to create modified mesh.

  3. Background • Copper nanoparticleshave shownto possess antimicrobial properties against bacteria such as Escherichia Coli and Bacillus subtilis(Faheem et al, 2011). • Past research has also shown that chlorhexidinedigluconate could kill both gram positive and gram negative bacteria (Chen et al., 2008).

  4. Rationale • Lack of access to drinking water • Current conventional methods have their disadvantages: • Non-controllable pore size • Lack of ability to control nanofiber composition/ functionalising agents for desired functions • Great demand for a more efficient and cheaper method of water purification.

  5. Objectives • Confirm the effectiveness of chlorhexidinedigluconate and copper nanoparticleselectrospun for the log removal of bacteria while integrated in separate nanofibre membranes • Determine the effectiveness of a combination of chlorhexidinedigluconate and copper nanoparticleselectrospun into the same membrane for water purification

  6. Hypothesis • A combination of copper nanoparticles and chlorhexidinedigluconate is more effective than the individual functionalising agents or no functionalising agents when electrospun into nanofibres.

  7. Variables • Controlled Variables: Ambientmeasurements, type and amount of bacteria and water, spinningparameters, size of mesh, methodsused for filtration and measurements • Independent Variable: Type(s) of anti-bacterial agent electrospunwithpolymer • Dependent Variable: The total log removal of colonyformingunits (CFUs)

  8. Materials • Aceticacid(18 M glacial) • Formicacid (98% concentration) • Nylon 6 ([-NH(CH2)5CO-], Sigma Aldrich) • Nutrient agar broth • Escherichia coli k-12 (ATTC) • Bacillusmegaterium(ATTC) • Sterilised water • Bleach

  9. Apparatus • Electrospinning apparatus • Petridishes • SterileSpreaders • Micropipettes • AA Spectrophotometer • Autoclave • Beakers • Buchner Funnel • Flask

  10. Preparation of Copper nanoparticles

  11. Preparation of Nanofibres (Daels et al., 2011)

  12. TestingwithBacteria

  13. TestingwithBacteria http://image.tutorvista.com/content/organic-compounds/filtration-buchner-funnel.jpeg

  14. Thank You

  15. Bibliography • Agarwal, S., Greiner, A., & Wendorff, J. H. (2008). Use of electrospinning technique for biomedical applications. Polymer, 49, 5603-5621. doi: 10.1016/j.polymer.2008.09.014 • Bhardwaj, N. & Kundu, S. C. (2010). Electrospinning: A fascinating fiber fabrication technique. Biotechnology Advances, 28, 325-347. doi: 10.1016/j.biotechadv.2010.01.004 • Bjorge, D., Daels, N., Devrieze, S. Dejans, P., Camp, T.V., Audenaer, W,... Van Hulle, S. W. H. (2009). Performance assessment of electrospunnanofibers for filter applications. Desalination, 249, 42-948. doi: 10.1016/.j.desal.2009.06.064 • Chen, L., Bromberg, L., Hatton, T. A., & Rutledge, G. C. (2008). Electrospun cellulose acetate. Fibers containing chlorhexidine as a bacteriacide. Polymer, 49, 1266-1275. doi: 10.1016/j.polymer.2008.01.003 • Daels, N., De Vrieze, S., Sampers, I., Decostere, B., Westbroek, P., Dumoulin, A.,...Van Hulle, S. W. H. (2011). Potential of a functionalisednanofibre microfiltration membrane as an antibacterial water filter. Desalination, 275, 285-290. doi: 10.1016/j.desal.2011.03.012 • Faheem, A.S. , Muzafar, A.K. , Saurabah, S, Chung, W.J. & Kim, H (2011). Polyurethane nanofibers containing copper nanoparticles as future materials. Applied Surface Science, 257, 3020-3026

  16. Bibliography • Frey, M. W., & Li, L. (2007). Electrospinning and porosity measurements of nylon-6/poly(ethylene oxide) blended nonwovens. Journal of Engineered Fibers and Fabrics, 2, 31-37. • Hem, R.P. , Dipendra, R.P. , Ki, T.N. , Baek, W.I. , Seong, T.H. & Kim, H.Y. (2011). Photocatalytic and antibacterial properties of a TiO2/nylon-6 electrospunnanocomposite mat containing silver nanoparticles. Journal of Hazardous Materials, 189, 456-471 • Lok, C.N. et al. (2006). Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteomic Research, 5(4): 916-24. • Rutledge, G. C. & Fridrikh, S. V. (2007). Formation of fibers by electrospinning. Advanced Drug Delivery Review, 59, 1384-1391. doi: 10.1016/j.addr.2007.04.020 • Sondi, I., Salopek-Sondi, B. (2004). Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science, 275, 177-182 • Y. Dzenis, Science 304 (2004) 1917. • Zussman, E., Theron, A. & Yarin, A. L. (2003). Formation of nanofiber crossbars in electrospinning. Appl Phys Let, 82, 973-5.

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