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Project Idea: Biofuels

Project Idea: Biofuels. Stanford iGEM 2009 Presentation by: Suzie + Robert 3/4/09. Biofuels Introduction. What makes a good biofuel?!?! Environmentally friendly Mass production - CHEAP! Chemically: High energy density Low water solubility. Biofuels Introduction.

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Project Idea: Biofuels

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  1. Project Idea: Biofuels Stanford iGEM 2009 Presentation by: Suzie + Robert 3/4/09

  2. Biofuels Introduction • What makes a good biofuel?!?! • Environmentally friendly • Mass production - CHEAP! • Chemically: • High energy density • Low water solubility

  3. Biofuels Introduction

  4. Biofuels Introduction • As for synthetic biology applications… • Most use E. coli or S. cerevisiae as a host to: 1. Engineer amino acid biosynthesis 2. Engineer fatty acid pathways 3. Engineer isoprenoid pathways

  5. Dr. James Liao

  6. Dr. James Liao

  7. 3-Methyl-1-Butanol

  8. 2-Methyl-1-Butanol

  9. Dr. James Liao

  10. Our Project • We would need to engineer a separate amino acid pathway to produce a possible biofuel • EX: Serine or alanine • Need to ID enzymes that can make this possible: • For Dr. Liao, it was KIVD (yeast) and ADH6 (lactococcus lactis)

  11. Five Possible Sub-projects 1. Synthesizers (constructing the pathway) 2. Riboregulators (controlling knockouts) 3. Modelers (Modeling for bottlenecks) 4. Bottleneckers (Building expression system) 5. ANY OF THE ABOVE

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