1 / 20

Build a bug activity

Build a bug activity. Introduction to Salmonella. What is a pathogen?. A pathogen is a disease causing agent. Fungus. Bacteria. Protozoa. Virus. Images: Janice Carr; CDC/Dr. Godon Roberstad; Wellcome Images; Anna Tanczos, Wellcome Images. Question.

faxon
Download Presentation

Build a bug activity

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Build a bug activity Introduction to Salmonella

  2. What is a pathogen? • A pathogen is a disease causing agent Fungus Bacteria Protozoa Virus Images: Janice Carr; CDC/Dr. Godon Roberstad; Wellcome Images; Anna Tanczos, Wellcome Images

  3. Question Can you name a disease caused by bacteria?

  4. Bacteria features Image: Genome Research Limited

  5. Salmonella • Salmonella infections can be transmitted between humans and animals. Infection via food is also possible. • This activity focuses on two types of Salmonella that cause two distinctive diseases: • gastroenteritis Salmonella • typhoid Salmonella. Image: David Goulding, Wellcome Trust Sanger Institute

  6. The genus: Salmonella Defined by biochemistry Defined by disease syndrome Affects Humans, cows & lizards Affects Humans only

  7. What’s the difference? SalmonellaTyphimurium SalmonellaTyphi Human specific Typhoid fever (systemic) 1 – 3 week incubation Symptoms Slow progressive fever (up to 40oC), sweating , abdominal pain. If left untreated can lead to intestinal haemorrhage or perforation. 601 unique genes • Generalist • Food poisoning / gastroenteritis (localised) • 6 – 24 hour incubation • Symptoms • Diarrhoea, vomiting and abdominal cramps • Zoonosis • 479 unique genes Image: David Goulding, Wellcome Trust Sanger Institute

  8. Antibiotic resistance Around the world, more drug resistance isolates of Salmonella Typhi are being found. Images: Genome Research Limited

  9. How does resistance occur? • Use of antibiotics creates a selective pressure. • Only bacteria with genes that confer resistance can survive a treatment of antibiotics. • Eventually they can make up the whole population. Population after antibiotics Before antibiotics Add antibiotics

  10. Horizontal transfer • New antibiotic genes can be acquired via horizontal transfer or conjugation. Images: Genome Research Limited

  11. New multi-drug resistant Salmonella Typhimurium • A new multi drug resistant strain of Salmonella Typhimurium is emerging in Sub Saharan Africa. • Sequencing the genome of the bacteria revealed important information about the biology of the organism. Image: David Goulding, Wellcome Trust Sanger Institute Hear from the leading scientist on the research programme

  12. The Activity • Research and assemble a Salmonella bacterial genome. • Use the information resources to research key genetic components which determine the bacteria’s disease causing ability. • Use modelling clay to create your bacterial genome.

  13. Information cards

  14. Your Genome Scholar Plasmid

  15. Complete worksheet

  16. Assemble a genome • Using modelling clay or the paper genome provided assemble three key genetic components on a chromosome backbone for either: • Salmonella Typhi or • Salmonella Typhimurium.

  17. Feed back your results • Add your results to the class spreadsheet or results table.

  18. Answer summary

  19. Answer Summary

  20. What’s the impact?

More Related