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Historical Perspective

International Glossina Genome Initiative (IGGI) Objectives, Accompolishments and Future Directions December 2006, Sanger Center, UK. Historical Perspective.

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Historical Perspective

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  1. International Glossina Genome Initiative (IGGI) Objectives, Accompolishments and Future DirectionsDecember 2006, Sanger Center, UK

  2. Historical Perspective • 2002-2004 - Strategic Research Activities of TRD (Molecular Entomology, Bioinformatics and Human African Trypanosomiasis) were deficient in tsetse-based activities for disease control. • A case was made to advance vector genomics to better understand disease dynamics and develop new or better vector control tools. • January 2004 - 1st Executive Committee • February 2005 - 2nd Executive Committee • December 2005 - 3rdExecutive Committee

  3. Missions accompolished: Genome size assessment has been completed

  4. Missions Accompolished:Preparatory genomics studies • Glossina (Gmm) EST projects Midgut, fat body and salivary glond ESTs Reproductive and developmental stage ESTs Antennae and head ESTs Full length cDNA library construction and seq • Palpalis EST projects Pilot midgut and fat body library Full length cDNA library construction and seq Antenna and head ESTs Symbiont genome sequences completed

  5. EST database summary

  6. BAC library Ordering info http://bacpac.chori.org/Of the 78 BACs 5’-end sequenced, there was no bacterial contamination. There were a few clones that weakly matched fly(Drosophila, Glossina, Musca) sequences, including a few that matchedGlossina RsaI repeats. Average size 138 kb. The sequencing was done without any problem, meaning that no region difficult to sequence and assemble contained in these BACs. The repetititve sequences have almost no similarity to those of Drosophila.

  7. VMRC29-36N9 Length: 152,111 bp Gmm-0829 GMsg-5904 GMsg-5538 Gmm-1240 Gmm-2211 Gmm-2908

  8. VMRC29-31O6 Length: 157,472 bp Gmm-3311 Gmm-3337

  9. VMRC29-3E11 Length: 142,153 bp Gmm-1479 Gmm-2698 Gmm-1755 Gmm-1756 GMsg-5383 GMsg-5866 GMsg-6214 GMsg-7527 GMsg-7635

  10. Advocacy for tsetse/trypanosome research ASTMH symposia Dec 05 - Burroughs Wellcome support

  11. Future plans set Dec ‘05 meeting Further genomics analysis Initiate physical map analysis Functional genomics Bioinformatics/genomics workshop Development of a Website Biological resources Advocacy for disease and genomics Capacity building plans

  12. Update on genomics activities • NIH holds a consultative meeting on future eukaryotic genome projects • Additional mosquito genomes • Additional pathogen genomes • Several other important vectors to be considered - • Tsetse (Gmm and possibly Gpp) given high priority • White paper submission to be planned

  13. Update on genomics activities • VectorBase launched • Currently housing An.gambiae, Ae. aegypti, Cu. pipiens and tick genomes • Undertake annotation activities • Display linkage analysis • Undertake and display functional genomics data (microarrays) - • Other genomes that can be integrated include tsetse, 2 sand flies, reduviid bug, louse

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