1 / 15

GENUS: NEISSERIA

GENUS: NEISSERIA. Amany Niazy Lab # 6. NEISSERIA SPECIES. Contains many species; 2 are human pathogens Neisseria gonorrhoea (gonococcus) Neisseria meningitidis ( meningococcus ) Other species are normal flora that inhabit the upper respiratory tract of humans. SPECIMEN.

jatin
Download Presentation

GENUS: NEISSERIA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. GENUS: NEISSERIA Amany Niazy Lab # 6

  2. NEISSERIA SPECIES • Contains many species; 2 are human pathogens • Neisseria gonorrhoea (gonococcus) • Neisseria meningitidis (meningococcus) • Other species are normal flora that inhabit the upper respiratory tract of humans.

  3. SPECIMEN • Neisseria gonorrhoea Urethral discharge  Genital swabs (servical or rectal) • Neisseria meningitidis CSF • Fastidious organism very sensitive to environmental factors Process specimen as soon as possible

  4. GRAM STAIN • Direct Gram Stain: • Neisseria are intracellular • organisms • GNDC inside and outside pus cells (PMN) (kidney or bean shaped)

  5. CULTURE • Inoculate into • Chocolate Agar • Basal media  at 40C (no solidification) add sheep blood • Keep in water bath at 70C to hemolyse blood • This releases and denatures many RBCs and serum proteins, • making them more readily digestible to the bacteria • Colonies appear: Colorless , raised, 1-2 mm in diameter

  6. CULTURE • Thayer Martin (selective medium) • Contain antibiotics to inhibit other bacteria and fungi VCNT Vancomycin, Colistin, Nystatin, Trimethoprim.

  7. INCUBATION • Temp 35-37 ˚C ONLY • Need 5-10% CO2 to grow: • CO2 envelop in anaerobic jar or • Candle jar • For 48 hours Non-pathogenic Neisseria grow at room temperature

  8. IDENTIFICATION • Gram stain  GNDC • Oxidase +ve • Carbohydrate utilization test

  9. IDENTIFICATION • Gram stain from plate  GNDC (Kidney shaped)

  10. IDENTIFICATION • All Neisseria species are Oxidase +ve • Preparation of Oxidase Test: • Add filter paper on a clean slide • Add 2-3 drops of oxidase reagent on filter paper • Use cover slip to transfer colony onto filter paper • + ve Purple color

  11. IDENTIFICATION • HISS medium. broth + serum + sugar + indicator (phenol red) Neisseria meningitides Neisseria gonorrhoeae

  12. YOUR WORK FOR TODAY • Prepare gram stain from culture. • Do oxidase test. • Subculture organism to chocolate agar. • Incubate in the CO2 jar. • Read after 48 hours. • See sugar utilization tube (demonstration) • See intracellular GNDC slide (demonstration slide)

  13. New diagnosis techniques: • Immunoassays • DNA hybridization • PCR • Doc Kaiser’s microbiology website http://student.ccbcmd.edu/~gkaiser/goshp.htm

  14. Numbers in Saudi Population 13 cases of meningococcal meningitis in 2007

  15. THANK YOU

More Related