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From making bread to making new DNA

From making bread to making new DNA. Methods in biotechnology. Traditional biotechnology methods. True or False. Traditional biotechnology was practiced thousands of years ago . Making yoghurt is not the result of a traditional biotechnological process .

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From making bread to making new DNA

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  1. Frommakingbread to making new DNA Methods in biotechnology

  2. Traditionalbiotechnologymethods

  3. True or False • Traditionalbiotechnologywaspracticedthousands of yearsago. • Makingyoghurtis not the result of a traditionalbiotechnologicalprocess. • Cheeseis made usingbacteria and/or fungi. • Purebreddogs are the result of traditionalbiotechnology.

  4. Traditionalbiotechnology Selective breeding The breeding of animals or plants that have desirable genetic traits (e.g. dairy cow). Hybridization The process of forming a hybrid by cross pollination of plants or by mating animals of different types. (e.g. wheat, corn).

  5. Fermentation The anaerobic breakdown of complex organic substances, especially carbohydrates, by micro-organisms, yielding energy.

  6. Yourturn Look for foodsproducedfromtraditional biotechnology in yourkitchen.

  7. Getting the desirablegenetics traits more quickly…….. Genetic engineering The technology that uses recombinant DNA , and other methods, to manipulate the genes of an organism.

  8. What is the difference? Traditional breeding differs from genetic engineering: • Traditional breeding is between the same or closely related species. • No control and hundreds of genes are worked on at a time. • Traditionalbreedingtakes a long time.

  9. Recombinant DNA technology Recombinant DNA technology is one of the key methods used in genetic engineering. Recombinant DNA technology The technology of producing a new, hybrid DNA from DNA coming from two different organisms. This new DNA does not exist naturally. It is genetic cutting and pasting!

  10. Basic steps in making recombinant DNA 1. Isolate DNA of interest. 2. Cut DNA with restriction enzymes to give DNA fragments or a specificgene. 3. Splice (paste) the DNA piece or geneinto a vector . This isyour recombinant DNA thatcanthen beused to transformcells, clone genes, make proteins, mapgenes, etc.

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