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1,3-Propanediol

1,3-Propanediol. Group 2 生科 陳麗如 生科 林嘉慧 資工 陳奎昊 資工 劉盈詮. Outlines. Set up genetic-modified experiment to produce 1,3-propanediol. Two ways to maximize yield of PDO. Linear programming Copasi. PDO 生合成：結構式. C 6 H 12 O 6 -> 1.5 C 3 H 8 O 2 + 1.5 CO 2.

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1,3-Propanediol

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  1. 1,3-Propanediol Group 2 生科 陳麗如 生科 林嘉慧 資工 陳奎昊 資工 劉盈詮

  2. Outlines • Set up genetic-modified experiment to produce 1,3-propanediol. • Two ways to maximize yield of PDO. • Linear programming • Copasi

  3. PDO 生合成：結構式 C6H12O6 -> 1.5 C3H8O2 + 1.5 CO2 Metabolic Engineering of Propanediol Pathways. Biotechnol. 1998, 14, 116-125

  4. Design specific primer pairs to amplify target genes glycerol dehydratase 1,3-propanediol dehydrogenase glycerol-3-phosphate dehydrogenase (NAD+) (dhaT) (dhaB) From Escherichia coli K-12 MG1655 Ins1 promoter Construct Ins1 promoter:G3P dehydrogenase –pTC vector Construct trc promoter:dhaB-dhaT-pTC vector Transform to E. coli K-12 Evaluate efficiency of PDO production

  5. Pathway Metabolic Engineering of Propanediol Pathways. Biotechnol. 1998, 14, 116-125

  6. Case 1 Anaerobic 1ATP Glucose 1 Propanediol + 1 Acetate + 1CO2 Metabolic Engineering of Propanediol Pathways. Biotechnol. 1998, 14, 116-125

  7. Experimental result from linear programming

  8. Simulation result from Copasi Conc. PDO:1 Time http://www.copasi.org/tiki-index.php

  9. Simulation result from Copasi (Cont.) PDO DHAP

  10. Case 2 Aerobic Glucose + 0.18 O2 1.45 Propanediol + 0.2 H2O +1.63 CO2

  11. Experimental Result fromLinear Programming

  12. Simulation Result from Copasi Conc. 1.45 Time

  13. Case 3 Aerobic 1ATP Glucose + 0.31 O2 1.42 Propanediol + 0.32 H2O +1.73 CO2 Metabolic Engineering of Propanediol Pathways. Biotechnol. 1998, 14, 116-125

  14. Experimental Result from Linear Programming

  15. Simulation Result from Copasi 1.42 Conc. Time

  16. Conclusions • Our proposed experiment resembles case1 (Anaerobic). • Case1 is not the best procedure to maximize the yeild of PDO. • The results of linear programming technique and Copasi tool are similar to biological data.

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