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H&E STAINING

H&E STAINING. Lab Lec # 4. What is H&E ?. The hematoxylin and eosin stain ( H&E) Use in histology and histopathology laboratories. ability to demonstrate a wide range of normal and abnormal cell and tissue components Benefit!

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H&E STAINING

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  1. H&E STAINING Lab Lec # 4

  2. What is H&E ? • The hematoxylin and eosin stain (H&E) • Use • in histology and histopathology laboratories. • ability to demonstrate a wide range of • normal and • abnormal cell and tissue components • Benefit! • it is a relatively simple stain to carry out on paraffin or frozen sections. • The first method of choice to determine whether a biopsy tissue is cancerous or not.

  3. How it works? • Hematoxylin • has a deep blue-purple color and • stains nucleic acids by a complex, incompletely understood reaction. • nuclei are stained blue, • Eosin • is pink and stains proteins nonspecifically. • cytoplasm and extracellular matrix have varying degrees of pink staining. • if abundant polyribosomesare present, the cytoplasm will have a distinct blue cast. • Well-fixed cells show considerable intranuclear detail. Nuclei show varying cell-type- and cancer-type-specific patterns of condensation of heterochromatin (hematoxylin staining) that are diagnostically very important.

  4. Procedure • Dewax • (paraffin wax –hyrdophobic/impervious to aqueous reagents) • Hydrate • (aqueous reagents can penetrate) • Add Hematoxylin • Add mordant (for binding) • Add alkaline solution (for more blueing) • Apply Eosin • Dehydrate & Mount

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