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General Pathology

General Pathology. Cellular and Organ Pathology Disorders of Glycogen Degradation. Pathology of Calcification. Jaroslava Dušková Inst. Pathol. ,1st Med. Faculty, Charles Univ. Prague. Disorders of Glycogen Degradation. Pathology of Calcification. Table of contents. Glycogen

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General Pathology

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  1. General Pathology Cellular and Organ Pathology Disorders of Glycogen Degradation. Pathology of Calcification. Jaroslava Dušková Inst. Pathol. ,1st Med. Faculty, Charles Univ. Prague

  2. Disorders of Glycogen Degradation. Pathology of Calcification.Table of contents • Glycogen • morphology, function, regulation • pathology states • inborn - glycogenoses • acquired • hyperglycemia – DM I, DM II, MODY • hypoglycemia – insulinoma • hyperglycemia – glucagonoma • glycogen storage in the neoplasms (clear cell kidney ca, seminoma, Ewing sarcoma… • Calcification • dystrophic • metastatic clinical manifestations complications

  3. Glycogen • linear and branched polymer • cca 60 000 - D-glucose molecules • monoparticles (beta) - muscle • complex particles (alpha) - hepatocyte

  4. Main Hormones in Glycogen Metabolism insulin glucagon

  5. The Actions of Insulin on Cells • Increased glycogen synthesis –in liver (and muscle) cells. Reducing high blood glucose levels in diabetes. • Forces adipose tissue to make fats; lack of insulin causes the reverse. • Decreased proteolysis • Decreased lipolysis • Decreased gluconeogenesis • Increased amino acid uptake • Increased potassium uptake • Arterial muscle tone – forces arterial wall muscle to relax, increasing blood flow, especially in micro arteries; lack of insulin reduces flow by allowing these muscles to contract

  6. Glucagon -physiologic effects • increase in blood concentration of glucose. Neurons can not utilize alternative energy sources like fatty acids • Glucagon stimulates breakdown of glycogen stored in the liver. • Glucagon activates hepatic gluconeogenesis. Non-hexose substrates such as amino acids are converted to glucose. • Glucagon also appears to have a minor effect of enhancing lipolysis of triglyceride in adipose tissue.

  7. Control of Glucagon Secretion • Secreted in response to hypoglycemia • Two other conditions : • Elevated blood levels of amino acids, after consumption of a protein-rich meal . Since high blood levels of amino acids also stimulate insulin release both insulin and glucagon are active. • Exercise: not clear whether the actual stimulus is exercise per se, or the accompanying exercise-induced depletion of glucose. • Negative control - glucagon secretion is inhibited by • high levels of blood glucose • insulin • somatostatin

  8. Glycogen Metabolism Diseases • diabetes mellitus type I (& LADA – late autoimmune diabetes of adults) - polygenic HLA-DR 3, 4 • diabetes mellitus type II - polygenic • MODY - more than 10 types described – monogenic • insulinoma • glucagonoma • insulin resistance in : • metabolic syndrome (=obesity, glucose intolerance, hypertension, hyperlipemia… ) • insulin receptor mutations • polycystic ovary syndrome • hypercortisolism…….

  9. Complications of Diabetes glucotoxicity due to: • formation of Advanced Glycation E products • release of pro-inflammatory cytokines and growth factors • generation of ROS (free radicals) in endothelia

  10. Complications of Diabetes • macroangiopathy • microangiopathy • retinopathy • nephropathy • neuropathy • infections….. diabetic gangrene

  11. EnzymesInvolved in Glycogen Metabolism • g.-synthase - brancher • phophorylase kinase - debrancher • g-6-phosphatase • -glucosidase

  12. Glucokinase role in maintenance of glucose homeostasis pancreas – B cell M ATP/ADP glucose – G6P K+ Ca2+ GLUT2 GKB insulin GKA glucose IR GKh GLUT2 glucose G6P glycogen hepatocyte

  13. Storage Diseases Def.: inborn errors of metabolism (mostly single gene abnormality) leading to an enzyme defect with subsequent accumulation of the substrate (& lack of the product) in tissues or organs„thesaurismoses“

  14. Glycogenosis I – von Gierke E defect - gl-6 - phosphatase Organ damage - liver, kidney

  15. Glycogenosis II – Pompe E -defect - alfa1,4-glycosidase Organ damage - heart

  16. Glycogen Storage Diseases -1.

  17. Glycogen Storage Diseases -2.

  18. Clear Intracellular Vacuoles& adjunct techniques • accumulations of water neg. • lipides SUDAN, OILRED • polysaccharides PAS, A-PAS

  19. Glycogen water soluble easily lost in long lasting water based fixative solutions

  20. Calcification Def.: depositions of Ca (mostly phosphate salts) in tissues or organs Classification: dystrophic metastatic

  21. Calcification - physiology • Matrix vesicles - osteoblasts- nidus calcification • Non collagen proteins - osteopontin, osteonektin, osteokalcin, Gla protein,sialoprotein • Alkalic phosphatase • Phospholipids • Collagen I • Hydroxyapatite

  22. Pathology Conditions with Calcium Deposits Calcification • dystrophic • metastatic Calcinosis • localized • generalized Chondrocalcinosis-pseudogout

  23. Calcification - Microscopy • Basophilic • Von Kossa - Ag impregnation • Alizarine red + • Tetracyclin fluorescence • Polarized light birefringence

  24. Dystrophic Serum Ca level: normal Tissues/Organs status dystrophic changes (necrosis, scar…, low metab. turnover) Metastatic Serum Ca level: Tissues/Organs status normal, local alcalisation (acid secretion - urine, stomach juice, sweat…) Calcification

  25. Dystrophic Calcification

  26. Dystrophic Serum Ca level: normal Tissues/Organs status dystrophic changes (necrosis, scar…, low metab. turnover) Metastatic Serum Ca level: Tissues/Organs status normal, local alcalisation (acid secretion - urine, stomach juice, sweat…) Calcification

  27. Ca Salts in the Calcified Foci • Ca phophate Ca3(PO4)2 • Ca diphosphate (Ca2P2O7) • Hydroxyapatite (Ca5 (PO4)3.OH

  28. Pathology Conditions with Calcium Deposits Calcification • dystrophic • metastatic Calcinosis • localized • generalized Chondrocalcinosis-pseudogout

  29. Dystrophic Calcification • Frequent • necrotic tissue • connective tissue • vessels • kalkospherites • intracellular calcifiction of lysosoms Less frequent tendon, cartilage, elastics, bas. membranes

  30. Calcinosis Forms - localised generalised Localisation– connective tissue, muscles

  31. Chondrocalcinosis crystal deposit disease (pseudogout) pyrophophate and hydroxyapatite deposits localisation– synovial membrane, cortilage, bone

  32. Calcium pyrophosphate deposition disease(Chondrocalcinosis, pseudogout) • Clinic: may simulate different diseases • Arthroscopy - chalky white deposits • Tophaceus deposits with crystalline aggregates • Crystals stain with von Kossa technique • Foreign body type multinucleated giant cell reaction • Deposits are in synovium, cartilage, bone

  33. Vitamin D3 (1,25(OH)2D3) is a Hormone • Vitamin D3 is not politically correct. • It discriminates depending where you live, the further you live from the equator the less sun exposure consequently the lower your Vitamin D3 level. • It discriminates against the elderly, as you age your skin loses up to 75% of its ability to make Vitamin D3. • It discriminates against the obese. They have lower levels of Vitamin D3, due to the fact it is oil soluble, that it builds up in the fat tissue. • It discriminates against skin color a dark complected person needs more sun exposure to produce their Vitamin D3 than a fair skinned person. • It also discriminates against a person who does as they are told. It has been reported the incident of breast cancers have increased 40% in Australia due to Vitamin D3 deficiency caused by sunscreen use.

  34. Metastatic calcification - causes • PTH secretion adenoma, carcinoma • destruction of bone – leukemia, myeloma, metastases, Paget (polyostotic) • vitamin D intoxication • renal failure – phophate retention - secondary hyperparathyreosis - PTH

  35. Pathology Conditions with Calcium Deposits Calcification • dystrophic • metastatic Calcinosis • localized • generalized Chondrocalcinosis–pseudogout Calciphylaxix – extensive microvascular calcification and occlusion / thrombosis

  36. Almafragi A, Vandorpe J, Dujardin K.Calciphylaxis in a cardiac patient without renal disease.Acta Cardiol. 2009 Feb;64(1):91-3. Calciphylaxis is a rare complication that occurs in 1% of patients with end-stage renal disease (ESRD) each year. Extensive microvascular calcification and occlusion/thrombosis lead to violaceous skin lesions, which progress to nonhealing ulcers with secondary infection, often leading to sepsis and death. The lower extremities are predominantly involved (roughly 90% of patients). Although most calciphylaxis patients have abnormalities of the calcium-phosphate axis or elevated levels of parathyroid hormone, these abnormalities do not appear to be fundamental to the pathophysiology of the disorder. We report on a case of histologically proven calciphylaxis in a 54-year-old woman with normal renal function and normal calcium-parathyroid homeostasis. She had a history of alcoholic cardiomyopathy, and was treated with warfarin anticoagulation. She has been successfully treated with antibiotics, i.v. biophosphonates and intensive local wound care. We recorded a complete wound healing in contrast to what is reported in other series.

  37. Calciphylaxis reported in: • hypoalbuminemia • malignant neoplasm • systemic corticosteroid use • anticoagulation with warfarin • chemotherapy • systemic inflammation • hepatic cirrhosis • obesity • rapid weight loss, and • infection….

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