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Evolutionary Genomics of Cytochrome P450 David R. Nelson University of Tennessee Dept. of Molecular Scie

Evolutionary Genomics of Cytochrome P450 David R. Nelson University of Tennessee Dept. of Molecular Sciences Center of Excellence in Genomics and Bioinformatics. 1107. 1547. 469. 483. 205. P450 distribution in major groups of organisms. 469 + 163 Sargasso Sea seqs.

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Evolutionary Genomics of Cytochrome P450 David R. Nelson University of Tennessee Dept. of Molecular Scie

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  1. Evolutionary Genomics of Cytochrome P450 David R. Nelson University of Tennessee Dept. of Molecular Sciences Center of Excellence in Genomics and Bioinformatics

  2. 1107 1547 469 483 205 P450 distribution in major groups of organisms

  3. 469 + 163 Sargasso Sea seqs. 55 1547 488 1107 80 70 6 Tree of Life

  4. Phytophthora ramorum Thalassiosira pseudonana

  5. Magnaporthe grisea Fusarium graminearum Neurospora crassa Saccharomyces cerevisiae Schizosaccharomycespombe CYP61 Phanerochaete (white rot) Fungi X Aspergillus nidulans Metazoa X Homo,Drosophila,C.elegans mouse, rat, Fugu, Anopheles Amoebozoa X Dictyosteliumdiscoideum (Slime molds) Eukaryotic Crown Group X Stramenopiles Diatoms, Kelp, Phytophthora (Heterokonts) - CYP51 Ciliophora Tetrahymena, Paramecium X Alveolates Apicomplexa Plasmodium (malaria) X Toxoplasma (animal parasites) CYP51 CYP97 Viridiplantae X Arabidopsis, Oryza (rice) Chlamydomonas, Poplar Rhodophyta X Cyanidioschyzon, Porphyra Plants Glaucophyta Cyanophora X Euglenozoa Trypanosoma, Leishmania Discicristata Heterolobosea Naegleria, Acrasis Diplomonadida Giardia lamblia Archaezoa Parabasala Trichomonas

  6. CYP21 11A1 11-Deoxycorticosterone 11B1 Corticosterone CYP17 CYP17 11B2 Aldosterone CYP21 CYP17 CYP17 11-Deoxdycortisol 11B1 CYP19 Cortisol

  7. Ecdysone metabolic pathway in insects From Niwa et al. J Biol Chem. Aug. 20 279, 35942-9 2004

  8. Brassinosteroid pathway CYP90D CYP90D CYP90C ROT3 CYP85A2

  9. P450 CLANS in plants 51 71 71, 73, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 89, 92, 93, 97 98, 99, 701, 703, 705, 706, 712, 719, 723, 726, 736 72, 709, 714, 715, 721, 734, 735 74 85, 87, 88, 90, 702, 707, 708, 716, 718, 720, 722, 724, 725, 728, 729, 733 86, 94, 96, 704, 730, 731, 732 97 710 711 727 727

  10. P450 distribution in plants, 1100 sequences in 62 families plus 550 Populus (cottonwood tree) P450s

  11. Collaborators: Rice/Arabidopsis Mary Schuler University of Illinois Soren Bak Copenhagen Suzanne Paquette University of Washington/ Copenhagen Daniele Werck-Reichhart Strasbourg

  12. freshwater Hydra

  13. Halocynthia roretzi adult amd larva Cnidarian Chordate Porifera (sponges) Suberites domuncula leech (annelid worm) plus hermit crab

  14. GATA-1 promoter/GFP transgenic zebrafish Buffer injected embryo under fluorescence CYP20 Morpholino injected under fluorescence CYP20 Morpholino injected under white light Data of Todd Penberthy, with permission

  15. X chromosome genes near the QTL peak

  16. Other significant QTLs that appeared in 2-5 experiments (see “http://drnelson.utmem.edu/qtl.map.html” for details) Cyp8b1 a cis QTL (peak on top of Cyp8b1) 5X Cyp17a1 has a QTL on Chr 16 at 23-38 Mb 5X Cyp2a4 has a QTL on Chr 2 at 103-116 Mb 5X Cyp2d9 has a QTL on Chr2 at 36-48 Mb 4X Cyp7b1 has a QTL on Chr 18 at 3-15 Mb 3X Cyp4v3 has a QTL on Chr 10 at 75-88 Mb 2X Cyp2c37 has a QTL on Chr 18 at 47-59 Mb 2X Cyp2c50 has a QTL on Chr 1 at 32-48 Mb 2X Cyp2s1 has a QTL on Chr 12 at 13-23 Mb 2X

  17. Fish human tree

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