'Incubate' presentation slideshows

Antibiotic Effects on Flavobacterium psychrophilum the cause of cold-water disease

Antibiotic Effects on Flavobacterium psychrophilum the cause of cold-water disease. Eric Wagner, Randy Oplinger Fisheries Experiment Station, UDWR Don Bone JP Egan State Fish Hatchery. Overview. In vitro tests with 4 antibiotics Penicillin toxicity tests

326 views • 17 slides

Southern Blotting

Southern Blotting . General Scheme for Southern Blot. Restriction Digest. Gel Electrophoresis . DNA Preparation: Denaturation/Depurination. Transfer to filter: Blotting. Detecting DNA: Probing. Southern Blot Analysis. Southern Blot. done. Restriction Digest of DNA Electrophoresis

2.5k views • 15 slides

Tian He 2008. 07. 06

Cultivation and Transformation of Yeasts. Tian He 2008. 07. 06. Media. non-selective: YPD without antibiotics Selective: Synthetic complete dropout medium (SC) (auxotroph) YPD with antibiotics. YPD: 1000 mL Yeast Extract 10 g Peptone (Tryptone) 20 g

432 views • 14 slides

Recombinant DNA Bacterial Transformation Student Instructions Ligation

Recombinant DNA Bacterial Transformation Student Instructions Ligation. Recombinant Transformation Ligation. Obtain reagent tubes labeled: - pAmp (digested) - pKan (digested) - 10X LB/ATP (ligation buffer) - T4 Lig (ligase)

300 views • 6 slides

Introduction:

335 views • 1 slides

Grind tissue in liquid N 2

Incubate @ RT X 5m. 7 ml Trizol/g tissue (use glass pipettes). Grind tissue in liquid N 2. vortex. Thaw on ice. Shake for 5m @ RT. Hand shake for 15s @ RT. 0.7ml CHCl 3 /ml Trizol. Spin @ 3500 krpm X 15m. Aqueous phase to 50 ml tubes. Measure approximate volume. organic phase.

132 views • 1 slides