'Restriction fragments' presentation slideshows

Biotechnology

Biotechnology. Introduction. Biotechnology is essentially the use of living organisms and their products for health, social or economic purposes.

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Amgen Bruce Wallace Transformation Labs (2-7)

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DNA Mapping and Brute Force Algorithms

DNA Mapping and Brute Force Algorithms. Outline. Restriction Enzymes Gel Electrophoresis Partial Digest Problem Brute Force Algorithm for Partial Digest Problem Branch and Bound Algorithm for Partial Digest Problem Double Digest Problem. Molecular Scissors.

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Outline

Outline. Last time: Molecular biology primer (sections 3.1-3.7) PCR Today: More basic techniques for manipulating DNA (Sec. 3.8) Cutting into shorter fragments Reading fragment lengths Reading DNA sequence Probing presence of specific fragments

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DNA Mapping and Brute Force Algorithms

DNA Mapping and Brute Force Algorithms. Outline. Restriction Enzymes Gel Electrophoresis Partial Digest Problem Brute Force Algorithm for Partial Digest Problem Branch and Bound Algorithm for Partial Digest Problem. Molecular Scissors. Molecular Cell Biology, 4 th edition.

643 views • 48 slides

Vacation slides from Bio 437

Vacation slides from Bio 437. Experiments. 1 Plasmid matching 2 Amplification & sequencing 3 Colorful mutations 4 Designer resistance 5 Southern bands 6 Clever cloning. Plasmid matching. Given 4 unlabeled plasmid samples and 4 plasmid maps match maps to samples

555 views • 42 slides

Recombinant DNA Technology

Recombinant DNA Technology. A gene must be isolated and well characterized before it can be used in genetic manipulations. One method of isolating and amplifying DNA of interest is to clone the gene by inserting it into a DNA molecule that serves as a vehicle or a vector .

4.72k views • 31 slides

pARA-R Recombinant

biotechnology lab program. pARA-R Recombinant. RFP Expression Sequence. Laboratory Protocols by:. Marty Ikkanda. Powerpoint by:. Anthony Daulo. Pierce College, Woodland Hills, CA. V.1.2.1. restriction analysis of pKAN-R and pARA. pKAN-R. 5408 bp. Bam H I. rfp 702 bp. pARA. 4058 bp.

266 views • 17 slides

pARA-R Recombinant

biotechnology lab program. pARA-R Recombinant. RFP Expression Sequence. Laboratory Protocols by:. Marty Ikkanda. Powerpoint by:. Anthony Daulo. Pierce College, Woodland Hills, CA. V.1.2.2. restriction analysis of pKAN-R and pARA. pKAN-R. 5408 bp. Bam H I. rfp 702 bp. pARA. 4058 bp.

298 views • 17 slides

Bacterial Transformation

Bacterial Transformation. RET Summer 2007. Overall Picture. Bio-Rad pGLO Transformation Insertion of GFP gene into HB101 E. coli. Transformation. The process of transferring foreign DNA fragments into a recipient (host) cell for growth and replication Our host cells: HB101 E. coli

510 views • 15 slides

pARA -R Restriction Digest: An Introduction to Plasmids and Restriction Enzymes

pARA -R Restriction Digest: An Introduction to Plasmids and Restriction Enzymes. Laboratory 2a. Overview. Purpose: Examine role of restriction endonucleses (enzymes) in genetic engineering Examine a bacterial plasmid and its use in biotechnology Methods:

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Lecture #9 Date _____

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Amgen Lab 2a & 4a

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Lecture #9 Date _____

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DNA Technology & Genomics

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The Case of the Crown Jewels: DNA in Forensics

The Case of the Crown Jewels: DNA in Forensics. Image from: http://climategate.tv/?tag=criminal-investigation. DNA: Individually Unique Molecules. Base pair sequences are unique for each person DNA contains the genes that determine individual characteristics

218 views • 7 slides

Background

Characterization of Salmonella enterica Subtypes by Optical Mapping Colin W Dykes OpGen Inc. 708 Quince Orchard Road, Gaithersburg, MD 20878. Background

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AUTOMATED FINGERPRINTING OF BAC LIBRARIES FOR THE CONSTRUCTION OF PHYSICAL MAPS OF LARGE GENOMES

AUTOMATED FINGERPRINTING OF BAC LIBRARIES FOR THE CONSTRUCTION OF PHYSICAL MAPS OF LARGE GENOMES M.-C. Luo 1 , C. Thomas 1 , Z. Xu 2 , H.-B. Zhang 2 , M. Malandro 3 , M. Morgante 4 , P., E. McGuire 5 , J. Dvorak 1

122 views • 1 slides