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Wx-7Am-5utr-F1a /Wx-7A-intr2-R1b

N4AT4D. N7AT7D. N7DT7B. CS. N4AT4D. N4AT4D. N7AT7D. N7DT7B. N7AT7D. N7DT7B. CS. CS. M. M. M. 2000 bp. 1420 bp. 1320 bp. 1650 bp. 1150 bp. 1000 bp. 850 bp. 650 bp. 500 bp. Wx-7Am-5utr-F1a /Wx-7A-intr2-R1b. Wx-5utr-F1c /Wx-4A-intr1-R1a. Wx-5utr-F2b /Wx-7D-intr1-R1a. A.

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Wx-7Am-5utr-F1a /Wx-7A-intr2-R1b

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  1. N4AT4D N7AT7D N7DT7B CS N4AT4D N4AT4D N7AT7D N7DT7B N7AT7D N7DT7B CS CS M M M 2000 bp 1420 bp 1320 bp 1650 bp 1150 bp 1000 bp 850 bp 650 bp 500 bp Wx-7Am-5utr-F1a /Wx-7A-intr2-R1b Wx-5utr-F1c /Wx-4A-intr1-R1a Wx-5utr-F2b /Wx-7D-intr1-R1a A B C Fig. S1 Genome-specific PCR amplification for the 5′-UTR and the first intron of the Wx genes. Each primer set was used to amplify Chinese Spring (CS, lane 1), N7AT7D (lane 2) and N4AT4D (lane 3) and N7DT7B (lane 4). M = 1 kb plus DNA ladder. PCR amplification with (A) 7A-specific primer set, (B) 4A (B genome)-specific primer set, and (C) 7D-specific primer set

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