Bismuth Sulphite Agar TM 039 - Technical Datasheet

1.        BISMUTH SULPHITE AGAR  Dehydrated Culture     TM Media​- Microbiology Culture Media Manufacturer and Exporter  904, 9th Floor, Bigjos Tower, Netaji Subhash Place, Delhi, 110034  Phone: +91-9999168770/ +91-11-71239900  Email Id: support.web@titanbiotechltd.com    Composition   Bismuth Sulphite Agar​ TM 039 for selective isolation of Salmonellae from faeces,  urine, sewage and other materials           

2. 1        Ingredients  Gms/Ltr.  Agar  20.00  Peptone  10.00  Bismuth sulphite  8.00  Beef extract  5.00  Dextrose  5.00  Disodium phosphate  4.00  Ferrous sulphate  0.30  Brilliant Green  0.025    * Dehydrated powder, hygroscopic in nature, stored in a dry place, in tightly-sealed  containers below 25°C and protected from direct Sunlight.  Instructions for Use  Dissolve 52.3gms in 1000ml of distilled water. Gently heat to boiling with gentle swirling  and dissolve the medium completely. ​DO NOT AUTOCLAVE​. Cool to 45-50°C prior to  dispense.  Appearance: ​Greenish yellow colour, solution opalescent with flocculent precipitate  pH (at 25°C): ​7.7 ± 0.2  Principle  Bismuth Sulphite Agar​ is used for the selective isolation of Salmonella spp. Salmonella  constitute the most taxonomically complex group of bacteria among Enterobacteriaceae.  Bismuth Sulphite Agar is a modification of Wilson and Blair formula. Also this medium  favors use of larger inoculum as compared to other selective media, as it has unique  inhibitory action towards gram-positive organisms and coliforms. Peptone and Beef extract  serve as sources as carbon, nitrogen, vitamins and essential growth factors. Disodium  phosphate acts as a buffering agent. Dextrose is a fermentable carbohydrate source of 

3. 2    the growth of microorganisms. Bismuth Sulphite Indicator and Brilliant Green are  complementary, inhibiting Gram-positive bacteria and coliforms, allowing Salmonella spp.  to grow. Ferrous Sulphate is used for H2S production. When H2S is present, the iron  incorporated in medium is precipitated, and positive cultures produce the characteristic  brown to black color with metallic sheen. Agar is the solidifying agent.  Interpretation   Cultural characteristics observed after inoculating (103 - 105CFU/ml), on incubation at  35-370C for 40 - 48 hours.    Microorganisms  ATCC  Inoculum  (CFU/ml)  Growth   Recovery  (%)   Appearance of  colony  Salmonella  enteritidis   13076   103 - 105  Good-luxuriant  >=50%  Black with metallic  sheen  Salmonella typhi   6539  103 - 105  Good-luxuriant  >=50%  Black with metallic  sheen  Escherichia coli   25922  103  None - poor  <=10%  Brown – green  (depends on the  inoculum density)  Shigella flexneri   12022  103  None - poor  <=10%  Brown  Enterobacter  aerogenes  13048  103  None - poor  <=10%  Brown – green    References   1. Andrews, W. H., G. A. June, P. S. Sherrod, T. S. Hammack, and R. M Amaguana.  Salmonella, p. 5.01-5.20. In FDA Bacteriological analytical manual, 8th ed. AOAC  International, Gaithersburg, MD. (1995).  2. Wilson, W. J., and E. M. Blair. A combination of bismuth and sodium sulphite  affording anenrichment and selective medium for the typhoid-paratyphoid groups  of bacteria. J. Pathol. Bacteriol.29:310. (1926).  3. Wilson, W. J., and E. M. Blair. Use of a glucose bismuth sulphite iron medium for the  isolation of B.typhosus and B. proteus. J. Hyg. 26:374-391. (1927). 

4. 3    4. Wilson, W. J., and E. M. Blair. Further experience of the bismuth sulphite media in  the isolation of B. typhosus and B. proteus. J. Hyg. 31:138-161. (1931).  5. Isenberg, H. D. (ed.). Clinical microbiology procedures handbook, vol. 1. American  Society for Microbiology, Washington, D.C. (1992).  6. Vanderzant, C., and D.F. Splittstoesser (eds.). Compendium of methods for the  microbiological examination of foods, 3rd ed. American Public Health Association,  Washington, D.C. (1992).  7. United States Pharmacopeial Convention. The United States pharmacopeia, 23rd ed.  The United States Pharmacopeial Convention, Rockville, MD. (1995).