1 / 19

Femtosecond dynamics and near-field microscopy of chemical and biological objects

Institute of Chemical Physics Moscow, Russ i a. Femtosecond dynamics and near-field microscopy of chemical and biological objects. A.N. Petrukhin, O.M. Sarkisov. Femtosecond dynamics of bifunctional molecules. Near-field microscopy of lipofuscin granules from human eyes.

wallis
Download Presentation

Femtosecond dynamics and near-field microscopy of chemical and biological objects

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Institute of Chemical Physics Moscow, Russia Femtosecond dynamics and near-field microscopy of chemical and biological objects A.N. Petrukhin, O.M. Sarkisov

  2. Femtosecond dynamics of bifunctional molecules. • Near-field microscopy of lipofuscin granules from human eyes.

  3. Photochromicreaction ESIPT reaction hn C-O bond cleavage: 250 - 400 fs Formation of intermediate X: 300-450 fs Formation of colored photoproduct (550-600 nm): 1 - 3 ps Excited state intramolecular proton transfer (ESIPT): < 80 fs Formation of twisted structure: 0.3 – 4.4 ps Formation of colored photoproduct (480-500 nm): 3.8 – 14 ps S.A. Antipin, A.N. Petrukhin, F.E. Gostev, V.S. Marevtsev,A.A. Titov, V.A. Barachevsky, Yu.P. Strokach, O.M.Sarkisov, Chem. Phys. Lett. 331 (2000) 378. F.E. Gostev,A.N. Petrukhin, A.A. Titov, V.A. Nadtochenko, A.V. Metelitsa, V.I. Minkin, O.M.Sarkisov, Rus. Chem. Bul.2004. 23 , 113-14.

  4. Bifunctional molecules: compounds 1-3 1: 2: 3: Does proton transfer take place?

  5. Experimental setup Pump: 405 nm, 40 fs Probe: Supercontinuum, 410-800 nm

  6. Photoinduced absorption dynamics

  7. Photoinduced absorption dynamics

  8. Product yield ratio dependence on pump energy Compounds 1,2 800 fs 3 ps ESIPT S1K* S1K S0K B S1** 120 fs 20 ps 400 fs S1* X B C-O cleavage S0K S0 S0K B B – photochromic product S0K – ESIPT product

  9. Product yield ratio dependence on pump energy Compound 3 21 ps 450 fs S2* X B B C-O cleavage 120 fs 850 fs 3 ps S1K* S1K S0K S1** S0K ESIPT S0 S0K B – photochromic product S0K– ESIPT product B

  10. Pump pulse chirp influence on wave packet motion Compound 1. Kinetic curve at 510 nm

  11. Pump pulse chirp influence on product yield ratio Time delay = 200 ps

  12. 2. Near-field microscopy of lipofuscin granules from human eyes.

  13. Near-field microscopy. Experimental Setup.

  14. 200 nm Topography Phase contrast Single LF granule. Atomic-force microscopy.

  15. Topography Transmission at 410 nm Single LF granule. Near-filed microscopy.

  16. Topography Fluorescence spectra Single LF granule. Near-filed microscopy.

  17. Two photon microscopy of LP granules

  18. Fluorescence decay of LH granules under 400 nm fs irradiation Fluorescence decay – epoxide formation?

  19. Prof. Oleg Sarkisov • Prof. Victor Nadtochenko • Andrey Titov • Fyodor Gostev • Andrey Petrukhin • Andrey Frolov • Alexander Sobennikov • Arseniy Aybushev • Artem Astafyev • Ivan Shelaev • Pavel Zolotavin Institute of Chemical Physics Moscow, Russia Our team: Thank you very much! Laser spectroscopy Lab femto.chph.ras.ru +7-095-939-7162

More Related