1 / 11

Tissue Engineering: Cell aggregates

Tissue Engineering: Cell aggregates. Thesis project Chemical and Process Engineering Ph.D. program ETSEQ Rovira i Virgili University. Student: Verónica Saravia Supervisor: Petros Lenas February 2005. What is “ Tissue engineering ”?.

yardan
Download Presentation

Tissue Engineering: Cell aggregates

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Tissue Engineering:Cell aggregates Thesis project Chemical and Process Engineering Ph.D. program ETSEQ Rovira i Virgili University Student: Verónica Saravia Supervisor: Petros Lenas February 2005

  2. What is “Tissue engineering”? • fundamental understanding of structure-function relationships in tissue • development of biological substitutes to restore, or improve tissue function IntroductionObjectives Methodology Future work

  3. IN VIVO • Metabolic heterogeneity • Cooperatively Introduction IntroductionObjectives Methodology Future work

  4. IN VITRO • 2-D Vs 3-D Cell aggregate COMPLEXITY Introduction IntroductionObjectives Methodology Future work

  5. LONG TERM OBJECTIVE • Synthesize in vitro part of the structural complexity of a tissue using cell aggregates and different systems configurations of increasing complexity. DEA SCOPE • Production of different cell aggregates • Characterization of the aggregates Introduction Objectives Methodology Future work

  6. Methodology: Aggregates production Assay Evaluation • Cells: HepG2 • Medium: DMEM +FBS +non essential AA +Glutamine + Anti-biotic • RWV reactor • Cell quantification • Viability • Glucose consumption • Geometry • Toxicology assays with APAP • Rotation speed • Culture time • Aggregation form • Toxicology results Introduction Objectives Methodology Future work

  7. Rotary Cell Culture System • Advantages: • low shear stress • high mass transfer rates Introduction Objectives Methodology Future work

  8. Responses: • Growth curve Variables: • Aggregate type • Culture conditions: • Rotation speed • Culture time • Viability • Geometry characteristics • Toxicity results Introduction Objectives Methodology Future work

  9. Toxicology test • Toxicant: • APAP ant-inflammatory drug hepatoxicity in vivo and in vitro • Assays: • cell quantification • cell viability • others Introduction Objectives Methodology Future work

  10. Future work Design, implementation and development of models of in vitro systems, using different systems configurations of increasing complexity. Introduction Objectives Methodology Future work

  11. Any questions... THANKS FOR YOUR ATTENTION!

More Related