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E18.5 (femur)

anti-obif. ALP staining. anti-obif. anti-collagen I. F. G. H. I. fibula. tibia. E18.5 (femur). E18.5 (hindlimb). primary Ab : anti-obif. primary Ab (-). P. kDa. WB : anti-obif. J. K. L. 75. anti-obif. M. N. O. 37. DAPI. MC3T3-E1 – day 0. – day 8. – day 14.

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E18.5 (femur)

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  1. anti-obif ALP staining anti-obif anti-collagen I F G H I fibula tibia E18.5 (femur) E18.5 (hindlimb) primary Ab : anti-obif primary Ab (-) P kDa WB : anti-obif J K L 75 anti-obif M N O 37 DAPI MC3T3-E1 – day 0 – day 8 – day 14 Induction (-) Day 23 – day 23 Calvarial cells -obif Calvarial cells – day 0

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