some details about Peptidomics analysis

1. Protein And Illness some details about Peptidomics analysis SUMO proteins are similar toPeptide Biomarkers, and SUMOylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. In contrast to ubiquitin, SUMO is not used to tag proteins for . Mature SUMO is produced when the last four amino acids of the C-terminus have been cleaved off to allow formation of an degrardtion between the C-terminal glycine residue of SUMO and an acceptor lysine on the target protein. Altmetric score Altmetric calculates a score based on the online attention an article receives. Each coloured thread in the circle represents a different type of online attention and the number in the centre is the Altmetric score. The score is calculated based on two main sources of online attention: social media and mainstream news media. Altmetric also tracks usage in online reference managers such as Mendeley and CiteULike, but these do not contribute to the score. Older articles will typically score higher because they have had more time to get noticed. To account for this, Altmetric has included the contCore 2 O-glycans terminated with sialyl-Lewis x (sLe x) are functionally important oligosaccharides that endow particular macromolecules with high-affinity glycan ligands for the selectin family. To date, antibodies that recognize these structures on leukocytes have not been described. We characterize such a monoclonal antibody (mAb) here (CHO-131). The binding specificity of CHO-131 was directly examined by means of synthetic glycopeptides containing precise O-glycan structures Abstract Sialic acids usually locate at the terminal of many glycan structures in either α(2,3) or α(2,6) linkage, playing different roles in various biological and pathological processes. Several linkage specific carboxyl derivatization methods have been reported to discriminate between α(2,3) and α(2,6)-linked sialic acids by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Among them, ethyl esterification was recently reported to achieve linkage specific derivatization between α(2,3) and α(2,6)-linked sialic acids with good selectivity. However, the method suffered from the instability of the generated lactones and byproducts of the derivatives. To overcome these shortcomings, a solid-phase two step derivatization method was introduced to convert the α(2,6)-linked sialic acid into ethyl esters and the α(2,3)-inked counterparts into N-methyl amides, respectively. Under the optimized derivatization conditions, our method was able to achieve accurate relative quantification of N-glycan linkage as well as their corresponding sialylated linkage types, superior to the ethyl esterification method. The solid phase derivatization strategy was further applied to investigate N-glycans from biosimilar antibody drug and human serum from patients and healthy volunteers. This method has the potential to be used in the biomarker discovery and pharmaceutical industry. Highlights • A novel acidic polysaccharide (designated LBP1B-S-2) was characterized from fruits of Lycium barbarumL.• Structures of LBP1B-S-2 and its sulfated derivative (Sul-LBP1B-S-2) were elucidated.

2. Sul-LBP1B-S-2 showed robust anti-angiogenesis effect on HMEC-1 cells without cytotoxicity. The effect of corn oil addition on mycelium growth and polysaccharide productions in the medicinal mushroom Ganoderma lucidum was studied. The results showed that when a level of 2% corn oil was added at the beginning of culture, the biomass and polysaccharide productions reached a maximum of 12.9 and 1.038 g/L, respectively, during 13-day cultivation. The pH variation along with morphology observation in culture provided an indirect inference to the promotional effect of oil addition. Moreover, a curve fitting analysis was carried out to assay the elevated effect on biomass and exopolysaccharide productions in oil added culture. The experimental data of substrates consumption and products formation in culture with oil addition were predicted through the fitting equations obtained in single carbon source culture. The numerical results further clarified the stimulatory effects of oil addition in G. lucidum culture.