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Impact of CMZ Treatment on p53 and p21 Levels in C6 Glioma Cells

This study investigates the effects of CMZ (20 µM) on p53 and p21 protein levels in C6 glioma cells, comparing untreated cells and those treated with DMSO as a control. Using immunoblot analysis, we observed minimal changes in the levels of both p53 and p21 after 16 hours of treatment. Equal amounts of protein from whole cell homogenates were analyzed on 12% SDS-polyacrylamide gels, yielding consistent results across two independent experiments. This suggests that CMZ does not significantly alter the expression of these critical regulatory proteins in C6 glioma cells.

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Impact of CMZ Treatment on p53 and p21 Levels in C6 Glioma Cells

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  1. Supplemental Figure 2. Little changes in the levels of p53 and p21 by CMZ. C6 glioma cells were untreated (lane 1) or treated with 20 µM CMZ (lane 2) or DMSO (lane 3) for 16 h before cell harvest. Equal amounts of the whole cell homogenates (30 µg protein/well of mini-gels) were separated on 12% SDS-polyacrylamide gels followed by immunoblot analysis using the specific antibody against p53 (top), p21 (middle), or actin (bottom). This result represents a typical result of two independent experiments.

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