1 / 17

Serology

Serology. Eva L. Dizon, M.D. Microbiology Our Lady of Fatima University. Serology. Antigen + Antibody. Immune Complex. Test that depends on the Formation of Immune Complex Nephelometry Immunodiffusion Complement fixation Cryoglobulins.

akamu
Download Presentation

Serology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Serology Eva L. Dizon, M.D. Microbiology Our Lady of Fatima University

  2. Serology Antigen + Antibody Immune Complex

  3. Test that depends on the Formation of Immune Complex Nephelometry Immunodiffusion Complement fixation Cryoglobulins Test in which Ag + Ab is fixed to a solid surface Agglutination Elisa Electrophoresis Serology

  4. Amount of Precipitine Antigen Excess (Postzone) Antibody Excess (Prozone) Equivalence Increasing Antigen Concentration

  5. Immunodiffusion A A A Present in reduce concentration I B B B Zone of equivalence + precipitation I Contaminant present in antigen I I

  6. Ouchterlony • Double diffusion • Characterize relationship between 2 antigen • If antigen are in the same precipitation line ,they fail to cross

  7. Ouchterlony Ag 1 Ag 2 Ag 4 Ab Limitations : Insensitive Needs large unit of Ab and Ag Resolve by CIE –placing agar in electric field Ag 3

  8. Nephelometry • IC in solution is monitored by spectrometry • Formation of IC is measured in a photoelectric cell as optical density Add increasing mount of antigen Ag Ag Ag

  9. Complement fixation test IC is fixed and consume complement 1. IC (ag +ab) + complement 2. IC +C +RBC------ no hemolysis (positive) Ag + ____ + C +RBC -------- hemolysis (negative)

  10. Cryoglobulins This are serum globulin that precipitate at T less than 37C To detect the presence in the blood ---- serum Samples are incubated in 4C for several hours ----- note for precipitation reaction Precipitated proteins isolated by centrifugation then assayed for IG by nephelometry,electrophoresis

  11. Ag and Ab fixed to a solid surface Agglutination test for the presence of antibody Indirect (Passive) Particle RBC Ag Ag Ag + Ab Ag Particle agglutination

  12. Neutralization test Hemmagglutination inhibition test Virus + RBC -------- Agglutinate Virus+ Ab + RBC -------- No Agglutination

  13. ASO titer Streptolysin O (Ag) + Serum of patient Agglutination If Ptx had Strep pyogenes infection Ab against Streptolysin O is present

  14. ELISA Direct – look for antigen Slide with Known ab ------ put serum ?Ag -------wash--------add enzyme labeled antibody--------wash--------look under spectrophotometer

  15. Elisa Indirect – look for antibody Slide with antigen --------+? Ab --------wash---------+ antihuman globulin -----------wash-------- look under spectrophotometer

  16. Fluoresence test Direct- looks for antigen 1. Slide put an antigen 2. Add fluorescein labeled antibody 3. Examine under the UV microscope Indirect- antibody Slide with known antigen Wash Add fluorescein labeled antihuman Ab

  17. Thank you and read your Manual TOO!

More Related