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Cellular Respiration: Harvesting Chemical Energy. Principles of energy conservation The process of cellular respiration Related metabolic processes 6O 2 +C 6 H 12 O 6 6H 2 O + 6CO 2 + energy. Concept 9.1. Metabolic pathways that release energy are called catabolic pathways
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Cellular Respiration:Harvesting Chemical Energy Principles of energy conservation The process of cellular respiration Related metabolic processes 6O2 +C6H 12O 6 6H2O + 6CO 2 + energy
Concept 9.1 Metabolic pathways that release energy are called catabolic pathways - fermentation and cellular respiration Fermentation: partial degradation of sugars that occurs w/out the help of O2 Cellular respiration: O2 is consumed as a reactant along w/ the sugar - more efficient
Redox Reactions • Reduction: gain of electrons • Oxidation: loss of electrons • Cellular respiration is a redox process
Redox Reactions Redox reactions release energy when electrons move closer to electronegative atoms - the relocation of electrons releases the energy stored in food molecules, and this energy is used to synthesize ATP
Concept 9.1 There is a transfer of one or more e- from one reactant to another; the electron transfers are called oxidation-reduction reactions or redox rxns. - the loss of e- from one substance is called oxidation - the addition of e- to another substance is called reduction
Concept 9.1 Electrons “fall” from organic molecules to oxygen during cellular respiration C6H12O6 + 6O2 6CO2 + 6H2O + Energy - by oxidizing glucose, cellular respiration takes energy out of storage and makes it available for ATP synthesis - carbohydrates and fats are reservoirs of electrons associated w/ hydrogen
Concept 9.1 The “fall” of electrons during respiration is stepwise, via NAD+ and an electron transport chain Glucose is broken down over a series of steps that are each catalyzed by a specific enzyme Hydrogen atoms are stripped from the glucose and usually passed to NAD+. - NAD+ is reduced in the rxn.
Concept 9.1 NAD+ is transformed to NADH - NADH will later be tapped to make ATP as the electrons continue their fall from NADH to oxygen Respiration uses an electron transport chain to break the fall of electrons to oxygen into several energy-releasing steps instead of one explosive rxn.
Concept 9.1 Electrons removed from food are shuttled by NADH to the “top” end of the chain. At the “bottom”, oxygen captures the electrons along with H+ ions to form water Food NADH ETC oxygen
Concept 9.1 Respiration consists of three stages: - glycolysis, the Krebs cycle, electron transport chain (ETC) Glycolysis breaks down 1 glucose into 2 molecules of pyruvate - occurs in the cytosol Krebs cycle breaks down pyruvate into CO2 - occurs in the mitochondrial matrix
Concept 9.1 ETC accepts electrons from the breakdown products of the first 2 stages - the energy released at each step of the chain is used to make ATP (oxidative phosphorylation); through redox rxns. oxidative phosphorylation accounts for 90% of generated ATP
Concept 9.1 Substrate-level phosphorylation: direct transfer of a phosphate to ADP by an enzyme Each molecule of glucose is degraded into carbon dioxide, water and 38 molecules of ATP
Coenzymes are intermediate energy carriers • Found in all cells • Assist enzymes in energy transfer • NAD and FAD • Join with H+ and e- • Many coenzymes are vitamins
Concept 9.1 Substrate-level phosphorylation: direct transfer of a phosphate to ADP by an enzyme Each molecule of glucose is degraded into carbon dioxide, water and 38 molecules of ATP
Cellular Respiration • Glycolysis • Krebs cycle • Electron transport chain
Concept 9.1 Cellular respiration occurs in the mitochondria Organic + O2 Carbon + H2O + Energy compounds dioxide C6H12O6 + 6O2 6CO2 + 6H2O + Energy 1 glucose = -686 kcals
Concept 9.2 Glycolysis means “splitting of sugar” - the 10 steps of glycolysis are broken down into two phases: energy investment and energy payoff - glucose (6C) 2 pyruvate (3C) Energy investment phase: the cell spends 2 ATP to phosphorylate the fuel molecules
Concept 9.2 Energy payoff phase: 4 ATP are produced by substrate-level phosphorylation; 2 NAD+ are reduced to 2 NADH by the oxidation of food Net energy yield: 2 ATP and 2 NADH
Glycolysis • Catabolic pathway • “To split sugar” • Glucose + 2ATP2 pyruvates + 4ATP + 2NADH • Occurs in cytosol • Anaerobic process • 10 step pathway
Concept 9.3 If O2 is present, energy stored in NADH can be converted to ATP Upon entering the mitochondrion, each pyruvate is first converted to a molecule of acetyl CoA (2C) - another NAD+ is reduced to NADH
Intermediate Reaction(Oxidative Decarboxylation) • Occurs if O2 is present • 2 Pyruvate2 CO2 + 2 NADH + 2 Acetate • Attaches acetate to CoA Acetyl CoA
Krebs Cycle • In mitochondrial matrix • Oxidation of acetyl CoA to CO2 • Products include: 2 ATP + 6 NADH + 2 FAD + 4 CO2 • 8 step pathway
Concept 9.3 Acetyl CoA will enter the Krebs cycle for further oxidation Krebs cycle - 8 steps, each catalyzed by a specific enzyme - Acetyl CoA (2C) enters, 2 CO2 (1C) leave, 3 NAD+ 3 NADH, 1 FAD 1 FADH2, 1 ADP 1 ATP
Concept 9.4 Cristae: inner membrane folding of the mitochondria - increases surface area for more ETC’s Electrons removed from food during gycolysis/Krebs are transferred by NADH to the first molecule of the ETC
The Electron Transport Chain • Embedded in the inner mitochondrial membrane • Most ETC molecules are proteins • Primary ATP generating pathway • Produces ATP via Substrate Level Phosphorylation and Chemiosmosis
Concept 9.4 Most of the electron carriers in the ETC are proteins called cytochromes (cyt). The process goes downhill with oxygen being the final e- acceptor - for every 2 NADH, 1 O2 molecule is reduced into 2 molecules of water
Concept 9.4 FADH2 adds its e- at a lower energy level than NADH on the ETC. -NADH = 3 ATP - FADH2 = 2 ATP ETC makes no ATP directly. It moves e- from food to oxygen breaking the energy drop to manageable amounts.
Concept 9.4 Inside the inner membrane are enzymes called ATP synthase. - makes ATP from ADP and a phosphate ATP synthase uses energy from the ion gradient to synthesize ATP. - proton gradient
Concept 9.4 The ETC is an energy converter that uses the exergonic flow of e- to pump H+ ions across the membrane - from the matrix to the inner membrane space. ATP synthases are the only place that are freely permeable to H+
Chemiosmosis: the energy coupling mechanism • Electron flow actively transports H+ from the matrix into the intermembrane space • Flow of H+ “down the gradient” thru the ATP synthase complex generates ATP
Concept 9.4 H+ gradient across a membrane couples the redox rxns. of the ETC to ATP synthesis - chemiosmosis: connection between the chemical rxn. Makes ATP and transport across a membrane
Concept 9.4 Chemiosmosis is also found in the chloroplasts - ATP is generated during photosynthesis - light drives both e- flow down the ETC and H+ gradient formation