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USER’S GUIDE

USER’S GUIDE . RIKEN Arabidopsis Transposon-tagged Mutant (RATM) Line Catalogue Database. START YOUR SEARCH. Select resource type psh : homozygous line pst : no information for zygosity. Enter information Resource Number: named by RIKEN BRC, used in MTA and order forms

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USER’S GUIDE

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  1. USER’S GUIDE RIKEN Arabidopsis Transposon-tagged Mutant (RATM) Line Catalogue Database

  2. START YOUR SEARCH Select resource type psh: homozygous line pst: no information for zygosity Enter information Resource Number: named by RIKEN BRC, used in MTA and order forms Line name: named by RIKEN GSC AGI(TAIR10) code: gene number of Arabidopsis annotated by TAIR Click here to start search

  3. CHECK CANDIDATE LINE LIST Gene(s) near the insertion site 5’: neighbor gene(s) predicted from the 5’-end flanking sequence 3’: neighbor gene(s) predicted from the 3’-end flanking sequence Candidate line(s) (click to open the information page) psh: homozygous line pst: no information for zygosity Line name (click to open the information page)

  4. FIND DATA FOR EACH LINE Line name Ds insertion site information predicted from 5’- and 3’-end flanking sequence Candidate Position: location on the corresponding chromosome 5’1(1)/3’1(1): AGI code of the nearest gene and the position of Ds insertion 5’1(2)/3’1(2): additional gene information when the location of Ds insertion is intergenic Some lines have two or more candidate positions for Ds insertion. This is because of the redundancy in the Arabidopsis genome. Additional candidate site (when applicable)

  5. GET MORE INFORMATION FROM RARGE DB (Optional, map position/flanking sequence available ) http://rarge-v2.psc.riken.jp/line Enter line name (Dsxx-xxxx-x) here RIKEN BRC confirms the insertion site before shipment. The information obtained from the analysis will be informed to the user.

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