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Bacillus

Bacillus. Bacillus sp. GP rod Spore former Examples B. anthracis B. cereus B. subtilis B. megatarium Exoenzymes Lipase Amylase Casease Gelatinase. Spores. Agar Characteristics. Starch Hydrolysis. Enzymes Amylase Maltase Substrate Starch Reaction Clear zone.

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Bacillus

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  1. Bacillus

  2. Bacillus sp. • GP rod • Spore former • Examples • B. anthracis • B. cereus • B. subtilis • B. megatarium • Exoenzymes • Lipase • Amylase • Casease • Gelatinase

  3. Spores

  4. Agar Characteristics

  5. Starch Hydrolysis • Enzymes • Amylase • Maltase • Substrate • Starch • Reaction • Clear zone

  6. Protein Hydrolysis • Enzymes • Proteases • Caseases • Substance • Casein • Reaction • Clear zone

  7. Lipid Hydrolysis • Enzymes • Lipase • Substrate • Tributyrin • Reaction • Clear zone

  8. Bacillus Tri-Plate

  9. Collagen Hydrolysis • Enzyme • Gelatinase • Use loop (vs. needle) • Inoculate several times • Stab deep • Substrate • Gelatin • Reaction • Clear Zone on Agar • Liquefy Gelatin in tube

  10. MSA • Selective • Differential • Substrate • Mannitol • Salt (7.5%) • pH = phenol red • Reaction • Growth • Fermentation

  11. Nitrate Reduction • Inoculate with loop • Incubate • Additional solutions • Sulfanilic acid (A) • Naphthylamine (B) • (+) = Red • Nitrate to Nitrite • No color, add Zinc • (+) = not red • (-) = red

  12. Citrate • Enzyme • Citrase • Substrate • Citrate • pH • Bromthymol blue • Reaction • Keto acids • Oxaloacetic • Pyruvate • Acetic acid • CO2 • CO2 + NaH2CO3 • Prussian blue = +

  13. Bile Esculin Agar (BEA) • Selective • Bile Salts • Differential • Esculin (CH2O) • Fe-NH3-citrate • Reaction • Growth in bile • Hydrolyze esculin • Esculetin-Iron Rxn • Black precipitate

  14. Results • Exoenzymes • Starch (amylase, maltase) • Lipid (lipase) • Proteins (Casease, protease) • Collagen (Gelatinase) • Reaction • (+) = clearing • (-) = no clearing

  15. Starch Hydrolysis

  16. Lipid Hydrolysis

  17. Protein Hydrolysis

  18. Gelatin (Collagen) Hydrolysis

  19. BEA and Citrate Results + + Bile Esculin Citrate

  20. Hydrolysis Results Summary casein lipid Gelatin Starch

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