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Effects of salt on growth of P. fluorescens

Effects of salt on growth of P. fluorescens. Alan Bohn and Liesel Hannes November 30, 2011 MB495 - Brown. How do salts affect growth of P. fluorescens ?. Common Anions and Cations Culture Density Lag phase Biphasic growth.

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Effects of salt on growth of P. fluorescens

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  1. Effects of salt on growth of P. fluorescens Alan Bohn and LieselHannes November 30, 2011 MB495 - Brown

  2. How do salts affect growth of P. fluorescens? • Common Anions and Cations • Culture Density • Lag phase • Biphasic growth

  3.  Culture density independent of salt choice at low concentrations NaCl

  4.  Magnesium has a negative effect as a cation Inhibits Maximum culture density and casues biphasic growth

  5.  Biphasic Growth not a artifact of overnight culture

  6.  Magnesium has a negative effect as a cation Inhibits maximum culture density and causes biphasic growth

  7.  Chloride ion in NaCl does not have a negative effect

  8.  Bromide ion has a severe negative effect

  9. Summary of Conclusions • Culture density independent of salt choice at low concentrations • Magnesium cation salts have a negative effect on culture density • Bromide anion has a negative effect on culture density

  10. Future Directions • Investigate the biphasic growth observed in MgSO4 and MgCl2 salts • Calculate ideal concentrations of the different salts • Test additional salts • Salt requirements under anaerobic conditions

  11. Questions?

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