OBJ 1-BIOACTIVITY MECHANISMS OF PROTECTIVE ACTION

1. FIGURE 1. W-1122 MILESTONES Year OBJ 1-BIOACTIVITY MECHANISMS OF PROTECTIVE ACTION i,. Anticarcinogenicity and antiviral effects -phytochemicals ii. Anti-obesity and anti-diabetes-phytochemicals iii. Anti-inflammatory/immune-enhancement - natural/induced chemical iv. Antimicrobial and anti-toxic phytochemicalsv. of phytochemicals OBJ 2- TOXICANT MECHANISMS AND BIOMARKERS i. Genotoxicity/carcinogenicity of phytochemicals and fungal toxins ii. Phytoestrogens and tumor growth iii. Immunotoxic effects of natural and induced chemicals OBJ 3- BIOACTIVE COMPOUND DISCOVERY i. Bioassay development/verification/application ii. Identification of novel bioactive compounds OBJ 4- MODULATION OF BIOACTIVITY AND TOXICITY BY FOOD MODIFCATION i. Bioassay development ii. Effects of food processing 0 1 2 3 4 5 Mechanistic animal feeding studies Dose response studies Mechanisms of action Cell culture / mechanisms Animal studies Animal -dose response, pure chemicals Animal - natural extracts Collaborative cell /animal studies Structure-function Microbial target Application to food Dose response studies in animals Biomarker identification/verification Microarray and mechanisms of action in cell culture Dose response studies Interactions and cancer therapies Mechanistic animal feeding studies Dose response studies Mechanisms of action Establish cell –based bioassays Structure-activity studies of known compounds Bioassay-directed fractionation of new compounds Identification/Analytical methods Validation in animals/humans Develop cell and animal- based bioassays Select bioactive chemical candidates Evaluate effects of food processing in animal models Validate in clinical studies