Slide 88, elastic artery (aorta), (10x obj.)

1. Slide 88, elastic artery (aorta), (10x obj.) TI elastic fibers TM TA

2. Slide 36, elastic artery (aorta), trichrome (10x obj.) NOT vasa vasorum TI elastic fibers TM TA

3. elastic fibers TM vasa vasorum TA Slide 36, elastic artery (aorta), trichrome (40x obj.)

4. Slide 30, muscular artery (4x obj.)

5. Slide 30, muscular artery (40x obj.) • Vessel Tunics • A. Tunica Intima • endothelium • subendothelium • connective tissue • smooth muscle • (longitudinal fibers) • internal elastic lamina • B. Tunica Media • vascular smooth muscle • external elastic lamina • (circular fibers) • C. Tunica Adventitia • connective tissue • (longitudinal)

6. Slide 92, muscular artery w/ intimal thickening (40x obj.) • Vessel Tunics • A. Tunica Intima • endothelium • subendothelium • connective tissue • smooth muscle • (longitudinal fibers) • internal elastic lamina • B. Tunica Media • vascular smooth muscle • external elastic lamina • (circular fibers) • C. Tunica Adventitia • connective tissue • (longitudinal)

7. Slide 95, muscular artery, trichrome (4x obj.)

8. Slide 95, muscular artery, trichrome (40x obj.) internal elastic lamina external elastic lamina tunica media tunica adventitia elastic fibers

9. Slide 95, medium vein, H&E (4x obj.)

10. Slide 95, medium vein, H&E (10x obj.) • Tunica adventitia • thicker than tunica media • larger veins have longitudinal smooth muscle in adventitia • Tunica media • 3-15 layers of VSMCs • no external elastic lamina • Tunica intima • endothelium • subendothelium • IEL generally not evident

11. tunica adventitia tunica media Slide 95, medium vein, trichrome (4x obj.)

12. Slide 95, medium vein, trichrome (10x obj.) elastic fibers Note: IEL sometimes evident w/ trichrome stain tunica media tunica adventitia

13. Slide 95, arteriole and venule, H&E (40x obj.) • Arterioles • Tunica intima • very thin subendothelial layer • generally lacking distinct internal elastic lamina • Tunica media • 1-3 layers of VSMCs • no external elastic lamina • Tunica adventitia • thin and ill-defined • Venules • Tunica intima • endothelium • pericytes • Tunica media • 1-2 sparse layers of VSMCs • no external elastic lamina • Tunica adventitia • thicker than tunica media

14. Slide 95, arterioles and venules, trichrome (40x obj.) venule arteriole

15. Slide 95, arteriole and venule (?), trichrome (40x obj.) venule? arteriole? Remember, the change in vessel size is gradual, so you’ll find intermediate sizes. To make classification easy, focus on vessels that are clearly one type or another.

16. Slide 95, arteriole and venule (?), trichrome (40x obj.) arteriole? (sectioned obliquely) venule?

17. Slide 95, small vein & artery, H&E (40x obj.) vein artery

18. Slide 95, small vein & artery, trichrome (40x obj.) vein artery

19. valve Note number of white blood cells in lumen Slide 30, lymphatic vessel (10x obj.)

20. Slide 40, trachea (10x obj.) Look in this layer for arterioles and venules

21. Slide 40, trachea (40x obj.) venule arteriole

22. Slide 102, capillaries in myocardium (40x obj.)

23. Slide 98, H&E (orientation) Look under endocardium to find Purkinje fibers chorda tendinae A-V valve leaflet papillary muscle atrial myocardium ventricular myocardium coronary vessels epicardium

24. Slide 98, H&E, (20x obj.) endocardium Purkinje fibers in “subendocardium” (note perinuclear space and large fiber size) myocardium cardiac muscle

25. Slide 98, H&E (orientation) Look under endocardium to find Purkinje fibers chorda tendinae A-V valve leaflet papillary muscle atrial myocardium ventricular myocardium coronary vessels epicardium

26. Slide 98, trichrome (20x obj.) endocardium Purkinje fibers (note perinuclear space and large fiber size) cardiac muscle

27. chorda tendinae papillary muscle A-V valve leaflet Note that distribution of elastic fibers faces blood flow. to atrium to ventricle Slide 98, trichrome (20x obj.)

28. Slide 99, H&E (orientation) aortic valve leaflet cardiac skeleton ventricular myocardium atrial myocardium A-V valve leaflet look here for A-V bundle of His

29. Slide 99, H&E (4x obj.) ventricular myocardium A-V bundle of His cardiac skeleton

30. Slide 99, H&E (20x obj.) A-V bundle of His cardiac skeleton

31. Slide 99, trichrome (orientation) aortic valve leaflet cardiac skeleton ventricular myocardium atrial myocardium A-V valve leaflet look here for A-V bundle of His

32. ventricular myocardium cardiac skeleton A-V bundle of His Slide 99, trichrome (4x obj.)

33. Slide 99, trichrome (20x obj.) A-V bundle of His cardiac skeleton

34. Slide 100, H&E (orientation) aortic valve leaflet ventricular myocardium atrial myocardium

35. Slide 100, Weigert (orientation) aortic valve leaflet ventricular myocardium atrial myocardium

36. Slide 100, Weigert (4x obj) to aorta aortic valve leaflet Note that distribution of elastic fibers faces blood flow. to ventricle

37. Slide 100, Weigert (4x obj) Note that distribution of elastic fibers faces blood flow. aortic valve leaflet aortic side of leaflet ventricular side of leaflet

38. Slide 102, H&E (orientation) coronary vessels epicardium ventricular myocardium atrial myocardium aortic valve leaflet