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Energy Costs

Genetic Enhancement of Biodiesel Feedstocks Richard F. Wilson Oilseeds & Bioscience Consulting, LLC Raleigh, NC. Energy Costs. Petroleum. Biofuel. Vegetable Oil Prices (¢/lb). Oil 2002 Current Peanut 32.5 65.6 Sunflower 23.3 58.1 Cotton 18.0 46.6

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Energy Costs

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  1. Genetic Enhancement of Biodiesel FeedstocksRichard F. WilsonOilseeds & Bioscience Consulting, LLCRaleigh, NC

  2. Energy Costs Petroleum Biofuel

  3. Vegetable Oil Prices (¢/lb) Oil 2002 Current Peanut 32.5 65.6 Sunflower 23.3 58.1 Cotton 18.0 46.6 Corn 19.2 43.6 Canola 20.5 47.7 Soybean 16.5 43.5 Palm 14.9 36.2 USDA FAS October 2007

  4. World Vegetable Oil Situation Growth Rate 2006/2007 MMT MMT/yr Supply 132 + 5.5 Demand 122 + 5.3 End Stocks 10 10 MMT Oil represents about 28 days supply World Use of Diesel Fuel = 321 billion gallons in 2007 Need 300 MMT of vegetable oil to make B20 Biodiesel USDA FAS Sept 2007

  5. How to Ensure Adequate Supply of Vegetable Oils? • Increase Crush Capacity • Increase Area of Production • Increase Yield per Area • Develop New Oil Crops • Genetically Enhance Oil Concentration & Quality in Major Oilseed Commodities

  6. Variation in Oil Concentration • Soybean 7.0 to 26% • Sunflower 9.0 to 53% • Canola 12 to 42% • Cotton 20 to 30% • Peanut 30 to 65% An increase from 20 to 30% oil in soybean seed equals a 50% gain in oil content per bushel

  7. Mapping & Marking Oil Genes

  8. Developing New Genomic Breeding Tools Genetic markers Genetic map QTL Bioinformatic Analyses Candidate Genes BAC contigs, ESTs Minimal Tiling Path & BAC-end sequences Physical Map New DNA markers in Physical Map

  9. Genetic Regulation of Oil Concentration

  10. Breeding for Increased Oil Concentration & Yielding Ability High Yield x High Oil ↓ F1 Plants ↓ F2 Population ↓SSD F3 Population ↓SSD F4 Population ↓Single Plants F4:5 Families ↓Select & Bulk Test for Yield & Oil ↓ Retest selections

  11. Genetic Enhancement of Oil % World Oil Supply, USDA FAS, Sept 2007

  12. Which one is Crude Peanut Oil?

  13. Oleic acid improves oxidative stability and ignition properties (cetane index) of biodiesel

  14. FAT B KAS II D9 DS FAD 2 FAD 3

  15. Linolenic Acid Pathway

  16. Genetic Regulation of Oleic Acid Concentration in Soybean

  17. MAS with Perfect Gene Markers GMFAD3Ahas 2 recessive alleles (aa29, aacx) GMFAD3Bhas1 recessive allele (bb29) GMFAD3Chas 2 recessive alleles (cccx, cc29) aa29 bb29 cc29 X aacx BB cccx 1% 18:3 3% 18:3 F1 F2 aa29bb29cccx aacx bb29 cc29 ~1% of the F3 generation Kristen Bilyeu, Columbia MO

  18. New Sources of Oleic Acid

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