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DNA RNA Protein Trait Protein Synthesis (Gene Expression) Notes

DNA RNA Protein Trait Protein Synthesis (Gene Expression) Notes Proteins (Review) Proteins make up all living materials. Proteins are composed of amino acids – there are 20 different amino acids

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DNA RNA Protein Trait Protein Synthesis (Gene Expression) Notes

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  1. DNA RNA Protein Trait Protein Synthesis (Gene Expression) Notes Proteins (Review) • Proteins make up all living materials

  2. Proteins are composed of amino acids – there are 20different amino acids • Different proteins are made by combiningthese 20 amino acids in different combinations

  3. Proteins are manufactured (made) by the ribosomes

  4. Function of proteins: • Help fight disease • Build new body tissue • 3.Enzymes used for digestion and other chemical reactions are proteins (Enzymes speed up the rate of a reaction) • 4. Component of all cell membranes

  5. Genes • Genes are the instruction manuals for our bodies. • They are the directions for building all the proteins that make our body function. • Genes are made of DNA.

  6. Gene Expression • Not all genes are active or expressed at the same time. • Why: Because the cell would produce molecules it did NOT need – waste of energy and raw materials

  7. Gene expression (protein synthesis) is when the product of a gene, or a specific protein is being produced by a cell. • some genes are – rarelyexpressed – adrenaline • some genes are – constantly expressed – hairgrowth, blood pressure • some genes are expressed sometimes and turned off others – estrogen.

  8. Making a Protein—Transcription • First Step:Copying of genetic information from DNA to RNA called Transcription Why? DNA has the genetic code for the protein that needs to be made, but proteins are made by the ribosomes—ribosomes are outside the nucleus in the cytoplasm. DNA is too large to leave the nucleus (double stranded), but RNA can leave the nucleus (single stranded).

  9. Nucleus

  10. Part of DNA temporarily unzips and is used as a template to assemble complementary nucleotides into messenger RNA (mRNA).

  11. Nucleus mRNA DNA DNA A U A T U A G C C

  12. mRNA then goes through the pores of the nucleus with the DNA code and attaches to the ribosome.

  13. Nucleus Original DNA mRNA Ribosome

  14. Making a Protein—Translation • Second Step:Decoding of mRNA into a protein is called Translation. • Transfer RNA (tRNA) carries amino acids from the cytoplasm to the ribosome.

  15. These amino acids come from the food we eat. Proteins we eat are broken down into individual amino acids and then simply rearranged into new proteins according to the needs and directions of our DNA.

  16. A series of three adjacent bases in an mRNA molecule codes for a specific amino acid—called a codon. • A triplet of nucleotides in tRNA that is complementary to the codon in mRNA—called an anticodon. • Each tRNA codes for a different amino acid. mRNA Amino acid tRNA Anticodon

  17. mRNA carrying the DNA instructions and tRNA carrying amino acids meet in the ribosomes.

  18. Amino acids are joined together to make a protein. Polypeptide = Protein

  19. Use one of the codon charts on the next page to find the amino acid sequence coded for by the following mRNA strands. CAC/CCA/UGG/UGA ___________/___________/___________/____________ AUG/AAC/GAC/UAA • ___________/___________/___________/____________

  20. 2nd Base 1st Base 3rd Base • CAC/CCA/UGG/UGA • ___________/___________/___________/____________ Histidine Proline Tryptophan Stop

  21. AUG/AAC/GAC/UAA • ___________/___________/___________/____________ Methionine Asparagine Aspartic Acid Stop

  22. Protein Synthesis

  23. Movie about translation at bottom of webpage. Click on hyperlink in picture above.

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