Innovations in Upper-Division Chemistry Laboratories at Regis University

2. Teaching innovations in upper-division instructional chemistry laboratories at Regis University Surendra N. Mahapatro, Department of Chemistry, Regis University, Denver, Colorado 80221.

3. Some of the Challenges Massive data collection with little insight Disconnect between upper division laboratories including physical, analytical, inorganic and biochemistry Relevance

4. Synthesis and Characterization Laboratory Spring 2014 • Synthesis and Characterization of Vandyl bis-acetylacetonoate Complex

5. Vandyl bis-acetylacetonoate Complex

6. Synthesis reported in 1957 insulin mimetic properties

8. Synthesis

9. IR: V= O stretch at 992.1 cm-1

10. UV-Vis Spectra

12. Crystal Field Diagram

13. H1NMR-Determination of Magnetic Susceptibility

14. EPR Spectrum of the Vanadyl-Complex

16. Magnetic Susceptibility Balance

17. 4 Fe2+-cytochrome c + 8 H+in + O2 4 Fe3+-cytochrome c + 2 H2O + 4 H+out Isolation, Purification and characterization of beef-heart cytochrome c oxidase Aerobic life

18. Retrieved from: http://www.bioscience.org/2004/v9/af/1237/fulltext.asp?bframe=figures.htm&doi=yes

19. Yoshikawa et al Science, 269, 1069-1074, 1995; ibid, Science,1996, 272, 1136-1144

20. Neurodegenerative diseases: Alzheimer’s and Parkinson’s Leigh syndrome (LS).

22. Cytochrome c Oxidase Heme analysis (nine micromoles of heme per gram of protein) Molecular activity (kinetics- spectrophotometry and oxygen electrode assay) Cyanide (suicide substrate) binding Subunit integrity- SDS-PAGE

23. Lenny Carruthers, PhD (UTHSCSA)Christina Closken, MD (Creighton) Baltazor Gomez , PhD (UTHSCSA) Natalie Gibson, PhD (UTHSCSA) Joaquin Urbina, PhD (KSU) Kenroy Crawford, PhD (Purdue) Anthony Giordion, PhD (Georgia Tech) Angello Aguilar; PhD (KSU) Aplolonio Aguilar, PhD (UT Austin) Heather Brungart, PhD (CU Boulder) Lisa Brassell, PhD (CU Boulder) Carissa Cawich, MD Hong T. Lee, DO (Turo, CA) Nhan Pham, DO (Turo College, CA) Ryan Farmer, MD (CU, Medical center) David Pinsinski, MD (Creighton) Clifford Everett, MD (George Town) Charles Tate , MD (Univ. Colorado) Karen Link, MS (UTHSC, San Antonio) Andrew Wallocot, PhD (Pen Sate) Elizabeth Forbes, MD (Univ. Colorado) Katie Srock, MD (Univ. Colorado) Regis alumni (Chemistry Majors) research associates

24. Jacob Garcia Micah Fernandez Andreina Lujan Sara Rodriguez Jennifer Campos Current undergraduate research associates

25. Research Corporation-Cottrell College science grant • ACS-PRF (type B) grant • NIH-AREA • NSF-ILI • Department of Energy-Rocky Flats • NREL –subcontract • Gates Foundation of Colorado-Partners in Science grant • Pittsburgh Memorial Analytical Conference: College Science grant program • Western Alliance to Expand student opportunities (WAESO); Hispanic Research Center, Arizona State University Grant Support

26. During the past 7 years, 32 students were supported by undergraduate research stipends. WAESO

27. Acknowledgement: • Prof. Michael Doyle, University of Maryland, College Park, Maryland • Fr. William T. Miller, S.J., Regis University, Denver, Colorado • Prof. Fraser Armstrong, Oxford • Prof. Duy Hua, KSU • Prof. Brindavan Ranu, Indian Association for the Cultivation of Science, Calcutta • Fullbright Foundation