Details-ATP synthase ICC in transfected 293T’s

Aug 24, 2014

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Details-ATP synthase ICC in transfected 293T’s. Confocal images taken at 60x, 2x zoom, 2x integration, and 2x fast scan. Green PMT=2000 for all images No image processing applied. Slide 1-negative control. Slide 2-IGFR parapoptosis. Slide 1-negative control. Slide 3-NK1R-non apoptotic death.

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Details-ATP synthase ICC in transfected 293T’s

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Details-ATP synthase ICC in transfected 293T’s • Confocal images taken at 60x, 2x zoom, 2x integration, and 2x fast scan. • Green PMT=2000 for all images • No image processing applied
Slide 1-negative control Slide 2-IGFR parapoptosis
Slide 1-negative control Slide 3-NK1R-non apoptotic death
Slide 1-negative control Slide 4-C9-apoptosis
Slide 2-IGFR parapoptosis Slide 3-NK1R-non apoptotic death
Slide 2-IGFR parapoptosis Slide 4-C9-apoptosis
Slide 3-NK1R-non apoptotic death Slide 4-C9-apoptosis

ICC

1.35k views • 116 slides

ATP?

ATP?. Depends on the system…. 1 ATP – Glucose to G-6-P   Ribose-5-P 1 ATP – But 2 high energy bonds to get to PRPP 4 ATP – In vertebrates to get onto IMP from PRPP 5 ATP – In prokaryotes to get onto IMP from PRPP

539 views • 25 slides

ATP

ATP. BOOM!. WHAT DO YOU DO?. WORK! Mechanical Chemical Transport . What do you look like?. Adenine. 3 Phosphate groups. Ribose. ATP!!!!!!. Bonds between P groups of ATP’s can be broken by hydrolysis (ADP) ATP + H2O  ADP + P What do you think ΔG is? – or +??? -7.3 kcal/ mol.

428 views • 15 slides

Isopropylmalate Synthase

Isopropylmalate Synthase. Amino acid analogs. Amino Acid Analogs. Analogs – resemble naturally occurring amino acids Inhibit Wild Type – Why? What happens if incorporated into protein? Why are normal amino acids not synthesized? . Expose Wild Type Salmonella to Fluoroleucine. Observed.

180 views • 5 slides

ATP

ATP. ATP. Adenosine Triphosphate referred to as the energy currency or energy carrier of living organisms NOT an energy storage molecule Nucleotide- comprised of a purine base ( adenine) attached to C1of a ribose sugar backbone and the phosphates are attached to C5. Structure of ATP.

363 views • 15 slides

ATP

11/17/2010. ATP. Controlled Energy Flow. What is the safest way to get out of a building 5 story building? Walking down stairs Jumping out the window Why?. Controlled Energy Flow. What is the safest way to get out of a building 5 story building? Walking down stairs

639 views • 24 slides

Figure S2. CA production in transfected 293T culture supernatant

Figure S1. Viral ratio in pair-wise growth competition assay. Values shown are the average taken from one representative experiment done in triplicate. The error bar represents the standard deviation between the triplicates . Figure S2. CA production in transfected 293T culture supernatant.

114 views • 2 slides

HMG CoA synthase

HMG CoA synthase. Philip A. Miller. Sequence. Mevalonate Pathway. Overall Structural Picture. Kinetics. - Difference in Rates with Mutation of Ala110Gly. Function. Electron density of the Catalytic loop. Important Amino Acids and their roles . Specific Amino Acid I ntegrations.

253 views • 13 slides

ATP

ATP. Controlled Energy Flow. What is the safest way to get out of a building 5 story building? Walking down stairs Jumping out the window Why?. Controlled Energy Flow. What is the safest way to get out of a 5 story building? Walking down stairs Jumping out the window.

496 views • 25 slides

ATP

ATP. Adenosine triphosphate- the principal chemical compound that cells use to store and release energy. Consists of adenine, Ribose, and three phosphate groups. ADP - adenosine diphosphate, it looks like ATP but has two phosphates instead of three.

726 views • 37 slides

293T

RNA oligoribonucleotides and cell transfections. miR-K1 mimic was synthesized by Sigma. The sequences were listed in Supplementary Table 1. Reverse transfection of RNA oligoribonucleotide (s) was done using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol.

178 views • 3 slides

ATP

Making energy!. ATP. The point is to make ATP !. Chemical energy. (a). First law of thermodynamics: Energy can be transferred or transformed but Neither created nor destroyed. For example, the chemical (potential) energy in food will be converted to the kinetic

639 views • 23 slides

ATP

ATP. The Energy Currency of Living Organisms. Objectives. Understand the molecule ATP. Expected outcome. Describe the structure of ATP Explain how ATP is broken down and synthesised Explain why ATP is known as the universal energy currency. Why do organisms need energy?.

385 views • 16 slides

ATP

Making energy!. ATP. The point is to make ATP !. The energy needs of life. Organisms are endergonic systems What do we need energy for? synthesis building biomolecules reproduction movement active transport temperature regulation. Where do we get the energy from?.

375 views • 13 slides

Anthranilate synthase- ASB2

Supplementary Figure-3. Tryptophan synthase-TSB type2. Anthranilate synthase- ASA1. Anthranilate synthase- ASA2. Anthranilate synthase- ASB1. Anthranilate synthase- ASB2. Tryptophan synthase-TSB type1.

147 views • 1 slides

Molecule of the Week ATP Synthase

Molecule of the Week ATP Synthase. ...a nanoscale machine. Two molecular motors / generators Enzyme Ion pump. Function: Synthesize ATP / Create proton gradient. ATP – Adenosine triphosphate. Cellular energy „currency“ DNA replication / transcription Cell signalling. ADP + P i → ATP.

357 views • 17 slides

Control ATP ATP g S AMP ADP phosphate

A. 400 300 200 100 0. Control ATP ATP g S AMP ADP phosphate. *. **. Fast-growing microbes (CFU, 10 5 /g-compost). 0. 2. 6. B. Incubation time (day). 12 9 6 3 0. *. Control ATP ATP g S AMP ADP phosphate. *. Actinomycetes (CFU, 10 5 /g-compost). 0. 2. 6.

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Prevalence of the Alkaliphile ATP Synthase Sequence Motif in Alkaliphilic Bacillus Strains

Prevalence of the Alkaliphile ATP Synthase Sequence Motif in Alkaliphilic Bacillus Strains Dr. Mack Ivey, Tanushree Thote Department of Biological Sciences, University of Arkansas.

151 views • 1 slides

2 ATP + 4 ATP = + 2 ATP

2 ATP + 4 ATP = + 2 ATP. So does this solve the direction problem? Only for a second … Where does this ATP come from, if we are E. coli growing in minimal medium… Glucose is the only carbon source. Need to make ATP from glucose, and this TAKES energy. Need only to regenerate ATP from ADP:.

758 views • 54 slides

ATP SYNTHASE

Cards retrieved from : http ://local.brookings.k12.sd.us/biology/teacherlinks/photosynactivities.htm. ATP SYNTHASE. ADENOSINE. P. P. ADENOSINE. P. P. ADENOSINE. P. P. ADENOSINE. P. P. ADENOSINE. P. P. P. P. P. P. P. P. ETC. ETC. ETC. ETC. CO 2. CO 2. CO 2. CO 2. CO 2.

531 views • 33 slides

ATP

ATP. Why do we use it for Energy?. ATP: Adenosine Triphosphate. Consists of Adensosine + 3 Phosphates Highly unstable molecule 3 phosphates each highly negative repel each other (like the wrong end of a magnet)

571 views • 31 slides

ATP

ATP. 1. Receptor Protein recieves Hormone 2. Hormone goes in 3. Transport vesicle floats 4. Hormone calls for info to be copied. 5. DNA copied to RNA 6. RNA leaves into the ER. 7. Ribosome reads RNA and builds Protein 8. Vesicle leaves ER for

183 views • 5 slides

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