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PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE

PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE. Ivanković A. 1 , Ramljak J. 1 , Šubara G. 2 , Kelava N. 1 , Prekalj G. 2. (1)   Department of Animal Production and Technology, Agricultural Faculty, University of Zagreb, Svetošimunska 25, 10 000 Zagreb, Croatia.

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PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE

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  1. PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE Ivanković A.1, Ramljak J.1, Šubara G.2, Kelava N.1, Prekalj G.2 (1)   Department of Animal Production and Technology, Agricultural Faculty, University of Zagreb, Svetošimunska 25, 10 000 Zagreb, Croatia. (2)   AZRRI – Agency for rural development of Istria Ltd. Pazin; Agenzia per lo sviluppo rurale dell’Istria Pisino

  2. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Introduction • autochthonous cattle breeds in Croatia • number of animals drastically declined → systematic upgrading • 1998. – 138 animals; 2008 – 460 animals • institutional measures (subsidies, promotions), established association (AZRRI), economic reaffirmations(brand “beef” production)

  3. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle • Aim • analyze of pedigree and genetic variability within Istrian Cattle → shaping breeding programmes and preserving strategies

  4. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Geographical location of Istrian Cattle populations Material & Methods • Pedigree information • data 2 151 (1994 – 2008.) • ENDOG v4.5 program • Blood samples • 51 unrelated animals • Fstat v2.9.3, GenePop 3.4., Bottleneck v1.2.02 program • 30 microsatellites belong to the panel of the MoDAD marker sets recommended by FAO

  5. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Results Generation interval and number of herds contributing sires (HS), grand-sires (HSS), great-grandsires (HSSS) in Istrian cattle.

  6. Number of alleles (MNA), observed and expected heterozygosity (Ho, HE), polymorphism information content (PIC), fixation coefficient (FIS) at 30 microsatellites. HEL9 9 0.647 0.663 0.641 0.034 ILSTS005 2 0.098 0.13 0.122 0.241 ILSTS006 9 0.627 0.636 0.603 0.003 INRA005 3 0.569 0.658 0.584 0.146 INRA023 8 0.667 0.822 0.798 0.166** INRA032 5 0.49 0.49 0.442 0.01 INRA035 4 0.451 0.471 0.428 0.053 INRA037 6 0.471 0.641 0.601 0.197** INRA063 5 0.49 0.614 0.535 0.195 MM12 8 0.784 0.725 0.688 -0.072 NCAM 6 0.471 0.537 0.513 0.116 TGLA122 7 0.686 0.746 0.715 0.053 TGLA126 5 0.706 0.764 0.725 0.086 TGLA227 10 0.784 0.842 0.824 0.079 TGLA53 11 0.588 0.766 0.737 0.177 Media 6.6 0.589 0.656 0.617 0.071*** Mean

  7. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Estimates of average inbreeding and the average relatedness in Istrian cattle

  8. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle • absence of bottleneck using Wilcoxon test (TPM)

  9. Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Conclusions • Istrian cattle → did preserve part of genetic diversity • presence of inbreeding, with absence of bottleneck • unsystematic pedigree data → DNA testing • still endangered breed → • continuous monitoring of population parameters, • adjusting breeding program • preserving Istrian cattle breeds have to have high national and international priority

  10. We thank all breeders and Reproduction Center for providing samples as well as Croatian Agriculture Agency.The study of Croatian breeds was partly supported by the Ministry of Science, Education and Sports, Croatia. Acknowledgements:

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