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Reaction Mechanism: Chicken Egg White Lysozyme

Reaction Mechanism: Chicken Egg White Lysozyme. Enzyme responsible for degrading bacterial cell walls Hydrolyzes the glycosidic linkage between NAM and NAG. Mechanism: Chicken Egg White Lysozyme. Substrate fits in groove in enzyme. Binding Site. Mechanism: Chicken Egg White Lysozyme.

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Reaction Mechanism: Chicken Egg White Lysozyme

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  1. Reaction Mechanism: Chicken Egg White Lysozyme • Enzyme responsible for degrading bacterial cell walls • Hydrolyzes the glycosidic linkage between NAM and NAG

  2. Mechanism: Chicken Egg White Lysozyme • Substrate fits in groove in enzyme Binding Site

  3. Mechanism: Chicken Egg White Lysozyme Glu35 acts as a Gen Acid, donating a proton to the glycosidic oxygen The carbocation intermediate is stabilized by Asp52 The oxygen from a water molecule attacks the carbocation, finishing the mechanism with reprotonation of Glu35

  4. Chapter EightLipids and Proteins Are Associated in Biological Membranes

  5. What is a Lipid • Lipids:a heterogeneous class of naturally occurring organic compounds classified together on the basis of common solubility properties • insoluble in water, but soluble in aprotic organic solvents including diethyl ether, chloroform, methylene chloride, and acetone • Amphipathic in nature • Lipids include: • Open Chain forms • fatty acids, triacylglycerols, sphingolipids, phosphoacylglycerols, glycolipids, • lipid-soluble vitamins • prostaglandins, leukotrienes, and thromboxanes • Cyclic forms • cholesterol, steroid hormones, and bile acids

  6. Fatty Acids • Fatty acid: an unbranched-chain carboxylic acid, most commonly of 12 - 20 carbons, derived from hydrolysis of animal fats, vegetable oils, or phosphodiacylglycerols of biological membranes • In the shorthand notation for fatty acids • the number of carbons and the number of double bonds in the chain are shown by two numbers, separated by a colon

  7. Fatty Acids (Cont’d) Length of fatty acid plays a role in its chemical character • Usually contain even numbers of carbons (can contain odd, depending on how they are biosynthesized) • FA that contain C=C, are unsaturated: If contain only C-C bonds, they are saturated

  8. Fatty Acids (Cont’d) • In most unsaturated fatty acids, the cis isomer predominates; the trans isomer is rare • Unsaturated fatty acids have lower melting points than their saturated counterparts; the greater the degree of unsaturation, the lower the melting point • Why is this?

  9. Triacylglycerols • Triacylglycerol (triglyceride):an ester of glycerol with three fatty acids • natural soaps are prepared by boiling triglycerides (animal fats or vegetable oils) with NaOH, in a reaction called saponification (Latin, sapo, soap)

  10. Soaps • Soaps form water-insoluble salts when used in water containing Ca(II), Mg(II), and Fe(III) ions (hard water) • The salt rinses off • Reactions with acids/bases as catalysts • Salts formed by Saponification • Base-catalyzed hydrolysis with salts formed

  11. Phosphoacylglycerols (Phospholipids) • When one alcohol group of glycerol is esterified by a phosphoric acid rather than by a carboxylic acid, phosphatidic acid produced • Phosphoacylglycerols (phosphoglycerides) are the second most abundant group of naturally occurring lipids, and they are found in plant and animal membranes

  12. Waxes • A complex mixture of esters of long-chain carboxylic acids and alcohols • Found as protective coatings for plants and animals Parrafin chains on either side of ester

  13. Sphingolipids • Contain sphingosine, a long-chain amino alcohol • Found in plants and animals • Abundant in nervous system • Has structural similarity to phospholipids • Ceramide tells cells to undergo apoptosis • Sphingosine tells cells to grow, divide and migrate Remove Phosphoethanolamine

  14. Glycolipids • Glycolipid: a compound in which a carbohydrate is bound to an -OH of the lipid • In most cases, sugar is either glucose or galactose • many glycolipids are derived from ceramides • Glycolipids with complex carbohydrate moiety that contains more than 3 sugars are known as gangliosides (Fig. 8.8, p. 207) Ceramide

  15. Steroids • Steroids: a group of lipids that have fused-ring structure of 3 six-membered rings, and 1 five-membered ring.

  16. Steroids

  17. Cholesterol • The steroid of most interest in our discussion of biological membranes is cholesterol

  18. Biological Membranes • Every cell has a cell membrane (plasma membrane) • Eukaryotic cells also have membrane-enclosed organelles (nuclei, mitochondria…etc) • Molecular basis of membrane structure is in lipid component(s): • polar head groups are in contact with the aqueous environment • nonpolar tails are buried within the bilayer • the major force driving the formation of lipid bilayers is hydrophobic interaction • the arrangement of hydrocarbon tails in the interior can be rigid (if rich in saturated fatty acids) or fluid (if rich in unsaturated fatty acids)

  19. Lipid Bilayers • The polar surface of the bilayer contains charged groups • The hydrophobic tails lie in the interior of the bilayer

  20. Biological Membranes • Plant membranes have a higher percentage of unsaturated fatty acids than animal membranes • The presence of cholesterol is characteristic of animal rather than plant membranes • Animal membranes are less fluid (more rigid) than plant membranes • The membranes of prokaryotes, which contain no appreciable amounts of steroids, are the most fluid

  21. Membrane Layers • Both inner and outer layers of bilayer contain mixtures of lipids • Compositions on inside and outside of lipid bilayer can be different • This is what distinguishes the layers

  22. Effect of Double Bonds on the Conformations of Fatty Acids • Kink in hydrocarbon chain • Causes disorder in packing against other chains • This disorder causes greater fluidity in membranes with cis-double bonds vs...... saturated FA chains

  23. Cholesterol reduces Fluidity • Presence of cholesterol reduces fluidity by stabilizing extended chain conformations of hydrocarbon tails of FA • Due to hydrophobic interactions

  24. Temperature Transition in Lipid Bilayer • With heat, membranes become more disordered; the transition temperature is higher for more rigid membranes; it is lower for less rigid membranes • Mobility of the lipid chains increases dramatically with increasing temperature. Why? What is happening?

  25. Membrane Proteins • Functions: transport substances across membranes; act as receptor sites, and sites of enzyme catalysis • Peripheral proteins (Protein 3 in figure below) • bound by electrostatic interactions • can be removed by raising the ionic strength (Why?) • Integral proteins (Proteins 1, 2 and 4 in figure below) • bound tightly to the interior of the membrane • can be removed by treatment with detergents or ultrasonification • removal generally denatures them (Why?)

  26. Proteins Can be Anchored to Membranes • N-myristoyl- and S-palmitoyl anchoring motifs • Anchors can be: • N-terminal Gly • Thioester linkage with Cys

  27. Fluid Mosaic Model • Fluid: there is lateral motion of components in the membrane; • proteins, for example, “float” in the membrane and can move along its plane • Mosaic: components in the membrane exist side-by-side as separate entities • the structure is that of a lipid bilayer with proteins, glycolipids, and steroids such as cholesterol embedded in it • no complexes, as for example, lipid-protein complexes, are formed

  28. Fluid Mosaic Model of Membrane Structure What benefits does this model provide to the cell?

  29. Membrane Function: Membrane Transport Passive transport • driven by a concentration gradient • simple diffusion: a molecule or ion moves through an opening • facilitated diffusion: a molecule or ion is carried across a membrane by a carrier/channel protein • Active transport • a substance is moved AGAINST a concentration gradient • primary active transport: transport is linked to the hydrolysis of ATP or other high-energy molecule; for example, the Na+/K+ ion pump • secondary active transport: driven by H+ gradient

  30. Passive Transport • Passive diffusion of species (uncharged) across membrane dependent on concentration and the presence of carrier protein

  31. 1˚ Active transport • Movement of molecules against a gradient directly linked to hydrolysis of high-energy yielding molecule (e.g. ATP)

  32. Membrane Receptors • Membrane receptors • generally oligomeric proteins • binding of a biologically active substance to a receptor initiates an action within the cell

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