How much money we need for a research project ?

1. How much money we need for a research project ? • Financial support is a must for undertaking any research work. • Unjustified request for fund may lead to rejection of the project • Too less of finance may lead to difficulty in completion of a proposed project. • Budget is a rational approach towards requirement of fund for research.

2. What is budget? • A budget ensures that sufficient resources (financial, personnel, and infrastructure /equipment) are available to complete the research work that is being proposed. • It should be based on a full analysis of research project costs, and aim to get the sponsor to pay for as many of those costs as possible, while ensuring that both the sponsoring agency and Institutional guidelines are followed.

3. Justification • The budget justification narrative, required by most agencies, is an important opportunity to describe exactly how budget items will be used to complete the study. • A carefully constructed detailed budget increases the likelihood of securing funding because it documents the research team’s expertise to the funding agency. An accurate budget is essential to provide the necessary resources to conduct the study.

4. At what stage it should be done? • Preparing a research proposal budget can seem like an overwhelming responsibility, especially for new investigators. • Preparing a detailed budget during the proposal stage can minimize budget management difficulties once the project is funded.

5. Budget of a research project

6. Nonrecurring Expenditure Refer to one time expenditure

7. Equipment for Sample processing

8. Equipment for Storage

9. Instruments for Quantification

10. Instruments for Quantification

11. Instruments for Quantification • Spectro flurometer (8-10L): Measurement of fluroescence. • Nephlometer (2-3L) : Immuno-turbidimetry • Chemiluminiscence (4-5L) : • Multimode reader (12-15L)

12. Instruments for Quantification Costly and big instruments • Gas Chromatography (15-20L) • HPLC (30-50L) • Atomic absorption spectrophotometer (30-40L) • Flowcytometer (50-60L) • Flowcytometer with cell sorter ( 75- 100L)

13. Equipments for Molecular Biology research • Thermo cycler personal (2-3L) gradient ( 5-6L) • Gel Electrophoresis (2-3 L) • Gel documentation System (5-6L) • Real time PCR (20-25 L) • Gene analysis system (40L)

15. BUDGET FOR EQUIPMENT*

16. Justification for the proposed equipment • The Department of Biochemistry, University College of Medical Sciences, Delhi, where the proposed research project work will be executed, has all the necessary equipments as mentioned in “facilities/equipments being extended by parent institution” except the above mentioned equipment. • A Real time PCR machine (Q-PCR), critical for quantifying gene expression patterns, is essential for achieving the objectives. • The department is initiating cell / tissue culture facility and have all the other equipments such as Laminer flow, CO2 incubator except an inverted microscope with a digital camera attached to a computer, for detailed image analysis. • We will also require liquid nitrogen containers that will be used for storing the cell lines. • A dedicated deep freezer will be essential for storing tissue culture material, temperature sensitive reagents and samples for the project as the existing freezers are overloaded.

17. Recurring Expenditure Repeating Expenditure • Manpower • Consumable • Travel • Contingency • Overhead • Expenditure to be mentioned Year wise separately for all the above mentioned items depending on the experimental protocol.

18. Manpower Research Personnel Help in planning, execution & data collection • Research associate ( 23-24 K*) • Medical officer ( 20K*) • Senior research fellow ( 18-20 K* ) • Junior Research Fellow ( 16-18 K* ) • Research Assistant ( 21K *) * + HRA @ as admissible

19. Technical Personnel • Technical Officer/ Computer programmer (29.5K*) • Data entry operator/ Statistician (26.5 K*) • Technician/ Data entry operator/ Nurse / Field worker ( 17.5*) • Lab. Assistant/ data entry operator Jr ( 15.5K*) • Office assistant/ Driver (11.9 K*) • Lab. Attendant/ Animal attendant ( 11K*) * Consolidated salary

20. BUDGET FOR SALARIES / WAGES

21. Justification for the manpower requirement (i) Junior Research Fellow (One) : The experiments and estimations planned in the proposed research project will be carried out by the investigators themselves / their post-graduate students. However, to assist in the basic work like isolation of cells / DNA, preparation of reagents, sample collection and processing and data computation, a skilled JRF is required. Therefore, we propose to recruit one JRF with M.Sc./M.Tech degree in Biochemistry/Life Sciences/Medical Laboratory Technology/ Biotechnology (NET qualified) with at least one year work experience in a Biochemistry/Molecular biology laboratory and in-hand experience in molecular biology techniques; pay as per DST rules. (ii) Laboratory attendant : The proposed project involves collection of large number of human samples. Transportation of samples from O.P.D. to lab, processing, storage, analysis, and disposal of samples, assisting tissue culture procedures like washing of plasticware and glassware, autoclaving and cleaning of lab accessories demands a full time helping hand. Therefore, we felt the need of a laboratory attendant with some work experience in laboratory work; consolidated pay as per DST rules.

22. Consumables

23. Consumables To be calculated year wise as per sample size and other variables. • Drugs /Medicines • Chemicals • Plastic wares/ glass wares • Disposables

24. Justification for consumables The budget for biological reagents and chemicals constitute a major expenditure for this project. Many of the reagents are not manufactured in India and have to be imported either directly or through their distributors. We will require reagents for (i) Gas chromatography (GC) based analysis, that include HPLC grade solvents, high quality nitrogen gas and standerds and GC vials. ECD column used for GC can cater to approximately 300 – 400 samples. As we will process more than seven hundred samples, we will require two such ECD columns (ii) Molecular biology work with special requirements for Real time PCR (Taqman master mix and real time tubes), reagents / kits for DNA and RNA isolation and PCR based reagents such as dNTPs, restriction enzymes, Taq polymerase for gene polymorphism studies (iii) Cell culture. For in vitro work, two cell lines will be commercially procured. (iv) As Real time PCR and cell culture are sensitive areas of research, we will require at least two sets of dedicated autopipettes exclusively for each area.

25. Plastic wares • Autopipette : Routine use (3-5K) For small volume (7-10K) Multichannel (20K) Electronic (50K) • Other commonly required plastic wares • ELISA plates, Microcentifuge tubes (2/1.5/0.5,0.2mL @ 1K/1000) , Microtips (1000/100/10uL @ 1k/1000), Tip boxes (1000/100/10uL @3K/12), centrifuge tubes (15/50mL @ 1K/100 ), sample storage boxes (2/1.5/0.5/0.2mL @ 3k/12), etc.

26. BUDGET FOR CONSUMABLE MATERIALS

27. Travel • Expenditure for travel include : • Sample collection ( particularly for survey/ epidemiologic/ multi centric sampling). • Travel expenditure for presenting research work in scientific meeting ( inland only). • Attending meeting for presenting annual reports to funding agency.

28. BUDGET FOR TRAVEL Justification for travel The budget for travel will be utilized for presentation of research findings in different scientific meetings and DST grant monitoring meetings.

29. Contingency • Expenditure under contingency include • Maintenance of equipment • Stationary • Printing material • Petty expenditure • Postage

30. BUDGET FOR OTHER COSTS / CONTINGENCIES

31. Overhead • A budgetary provision by the funding authority for infrastructural expenses to the Investigator & Project executing Institute. • Electrical fixture, AC, refrigerator, furniture, telephone/ internet bill, common minor equipment, contingent salary for manpower assistance, gas etc. • Calculated in terms of percentage of the total budget excluding equipments.

32. BUDGET ESTIMATES: SUMMARY

33. Some funded projects by various funding agency • The effect of low glycemic composite flour on diabetic and normal subjects • Pharmacokinetic Profile of Nasal Corticosteroid and Oral antiHistaminic used in combination for treatment of Allergic Rhinitis in rural India. • Genotype phenotype correlation in Wilson's Disease within families

34. Study of New Gemostatic Gene Polymorphisms in Young Patients from North India with acute Myocardial Infraction • Genetic, Metabolic and Hormonal Profile in Obstructive Sleep Apnea Syndrome • Invitro inhibition of T cell Cytokines production by methotrexate: Can it predict response to methotrexate in patients with rheumatoid arthritis • Gene-Environment interactions and susceptibility to breast cancer.

35. Some ICMR funded Project • MOLECULAR BASIS OF AGE-RELATED DECLINE IN LEARNING AND MEMORY ;M.S.KANUNGO BANARAS HINDU UNIVERSITY, VARANASI   • SELENOPROTEINS AND ANTIOXIDANTS IN PATHOGENESIS OF ALZHEIMER'S DISEASE : P.RANI, P.S.G.COLLEGE OF TECHNOLOGY, COIMBATORE • PATTERNS OF FOOD ALLERGEN- SPECIFIC CYTOKINE PRODUCTION BY T LYMPHOCYTE SUBSETS OF ATOPIC CHILDREN : B.P.CHATTERJEE INDIAN ASSOCIATION FOR CULTIVATION OF SCIENCE ,KOLKATA • A STUDY ON THE NOVEL ANALGESIC EFFECT OF INTRASPINAL LOPERAMIDE IN A MURINE MODEL OF POSTSURGICAL PAIN : S.K.B.RAY, ALL INDIA INSTITUTE OF MEDICAL SCIENCES ,NEW DELHI   •   IS OXIDATIVE STRESS AND LIPID PEROXIDATION AN VIABLE MECHANISM OF PERPETUATION OF VASCULAR PATHOLOGY DUE TO HYPERHOMOCYSTEINEMIA?   SEEMA BHARGAVA, SIR GANGARAM HOSPITAL , NEW DELHI

36. ICMR funded project • EVALUATION OF PLATELET RECEPTOR POLYMORPHISM IN PATIENTS WITH CORONARY ARTERY DISEASE, ENU SAXENA, ALL INDIA INSTITUTE OF MEDICAL SCIENCES , NEW DELHI   • ESTABLISHMENT OF MOLECULAR CHARACTERIZATION OF HEMOLOGLO- BINOPATHIES AND PRENATAL DIAGNOSIS OF THALASSEMIA AND SICKLE CELL DISEASE AT REGIONAL CENTRES ,M.K.GHOSH,N.R.S.MEDICAL COLLEGE AND HOSPITAL,KOLKATA   • EVALUATION OF THE BENEFICIAL EFFECTS OF TAURINE SUPPLEMENTATION IN PATIENTS WITH DIABETES MELLITUS ,S.SETHUPATHY,RAJAH MUTHIAH MEDICAL COLLEGE AND HOSPITAL, ANNAMALAI NAGAR  

37. Title of the Project : Association of Common Variants of Inflammatory Cytokine Genes with Risk of Nephropathy in Type 2 Diabetes among Asian Indians Objective : Eight single nucleotide polymorphisms (SNPs) of pro-inflammatory cytokine genes (CCL2, TGFB1, IL8, CCR5, and MMP9) were to be genotyped. Sample : Two independently ascertained type 2 diabetic cohorts with nephropathy (n=200), without nephropathy (n=200) and control ( n=200).

38. Methodology: • DNA will be isolated by DNA isolation kit. • Genotyping will be carried out by PCR- RFLP using a thermocycler. • hsCRP will be estimated by ELISA using commercially available kit. ( Note: Collaborative dept. have ELISA reader & do not have a thermocycler. 50 sample DNA isolation kit costs = Rs 5 K, 96 well hsCRP kit costs Rs 12K)