120 likes | 143 Views
Genetics and Biotechnology. 13.2 DNA Technology. Genetic Engineering -manipulating the DNA of one organism in order to insert DNA from another organism. Genetics and Biotechnology. Applications of recombinant DNA. Bacteria: used to produce hormones and antibiotics
E N D
Genetics and Biotechnology 13.2 DNA Technology Genetic Engineering-manipulating the DNA of one organism in order to insert DNA from another organism.
Applications of recombinant DNA • Bacteria: used to produce hormones and antibiotics • Plants: made to be resistant to herbicides • Animals: replacement of genes which cause disorders (gene therapy); cows could produce medicine in milk
Genetics and Biotechnology Transgenic Organisms Biotechnology • Organisms, genetically engineered by inserting a gene from another organism, are called transgenic organisms.
Genetics and Biotechnology Restriction Enzymes • EcoRI-Restriction enzymespecifically cuts DNA containing the sequence GAATTC. • The ends of the DNA fragments, called sticky ends • DNA fragments cut by restriction enzymes can pair up and join with any other DNA fragments cut by the same enzyme
Genetics and Biotechnology Gel Electrophoresis • Anelectric current is used to separate DNA fragments according to the size of the fragments in a process called gel electrophoresis. • When an electric current is applied, the negatively charged DNA fragments move toward the positive end of the gel. • The smaller fragments move farther faster than the larger ones.
A technique aimed at correcting mutated genes that cause human diseases is called gene therapy. Genetics and Biotechnology • Scientists insert a normal gene into a chromosome to replace a dysfunctional gene. • Genomics is the study of an organism’s genome.
Genetics and Biotechnology 13.2 DNA Technology DNA Tools • Genome total DNA in the nucleus of each cell. Transgenic Tobacco Plant with Firefly gene
Genetics and Biotechnology PCR • Polymerase chain reaction (PCR) atechnique for copying a piece of DNA a billion-fold. • The process creates a chain of many nucleotides, and the chain is a strand of DNA. • Used in genetic fingerprinting
Genetics and Biotechnology 13.2 DNA Technology