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CHMI 2227E Biochemistry I

CHMI 2227E Biochemistry I. Nucleic acids: replication. DNA. Nucleus. DNA replication. 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’. 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’. 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’. DNA replication. DNA replication.

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CHMI 2227E Biochemistry I

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  1. CHMI 2227EBiochemistry I Nucleic acids: replication CHMI 2227 - E.R. Gauthier, Ph.D.

  2. DNA Nucleus DNA replication CHMI 2227 - E.R. Gauthier, Ph.D.

  3. 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’ 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’ 5’AGCTAGCTGATATCGCGATCG3’ 3’TGCATCGACTATAGCGCTAGC5’ DNA replication CHMI 2227 - E.R. Gauthier, Ph.D.

  4. DNA replication CHMI 2227 - E.R. Gauthier, Ph.D.

  5. Non-conservative Conservative Dispersive Semi-conservative DNA replication1) Conservation of the parental strands CHMI 2227 - E.R. Gauthier, Ph.D.

  6. PNAS 1958;44;671-682 DNA replication1) The Meselson and Stahl experiment CHMI 2227 - E.R. Gauthier, Ph.D.

  7. Replication forks DNA replication2) Directionality of replication CHMI 2227 - E.R. Gauthier, Ph.D.

  8. 5’ 5’ Replication fork 3’ 5’ 3’ Direction of the replication fork 3’ 5’ DNA replicationThe replication fork 3’ CHMI 2227 - E.R. Gauthier, Ph.D.

  9. DNA replicationLarge DNA molecules can have multiple origins of replication CHMI 2227 - E.R. Gauthier, Ph.D.

  10. DNA replication2) Directionality of replication 3’ 5’ 5’ 3’ 3’ 5’ 5’ 3’ CHMI 2227 - E.R. Gauthier, Ph.D.

  11. DNA replication2) Directionality of replication CHMI 2227 - E.R. Gauthier, Ph.D.

  12. DNA replication2) Directionality: Okazaki fragments CHMI 2227 - E.R. Gauthier, Ph.D.

  13. DNA replicationThe replication fork CHMI 2227 - E.R. Gauthier, Ph.D.

  14. DNA replication3) Nature of the replication machinery CHMI 2227 - E.R. Gauthier, Ph.D.

  15. DNA replication3) DNA polymerase CHMI 2227 - E.R. Gauthier, Ph.D.

  16. DNA replication3) DNA polymerase CHMI 2227 - E.R. Gauthier, Ph.D.

  17. DNA replication3) DNA polymerase CHMI 2227 - E.R. Gauthier, Ph.D.

  18. DNA replication3) DNA polymerase III of E. coli CHMI 2227 - E.R. Gauthier, Ph.D.

  19. (Clamp loader) (DNA polymerase) DNA replication3) DNA polymerase III of E. coli CHMI 2227 - E.R. Gauthier, Ph.D.

  20. DNA replication3) DNA polymerase III of E. coli http://oregonstate.edu/instruction/bb492/figletters/FigG1.html CHMI 2227 - E.R. Gauthier, Ph.D.

  21. Put DNA Here! Direction of replication Sliding clamp DNA replication3) DNA polymerase III of E. coli CHMI 2227 - E.R. Gauthier, Ph.D.

  22. DNA replication3) Primase CHMI 2227 - E.R. Gauthier, Ph.D.

  23. DNA replicationInitiation at the origin of replication CHMI 2227 - E.R. Gauthier, Ph.D.

  24. Unwinds DNA http://oregonstate.edu/instruction/bb492/figletters/FigH4.html DNA replicationLeading strand synthesis CHMI 2227 - E.R. Gauthier, Ph.D.

  25. DNA replicationLagging strand synthesis CHMI 2227 - E.R. Gauthier, Ph.D.

  26. DNA replicationLagging strand CHMI 2227 - E.R. Gauthier, Ph.D.

  27. Lacking Phosphodiester bond DNA replication - DNA ligase CHMI 2227 - E.R. Gauthier, Ph.D.

  28. DNA replication4) Proofreading CHMI 2227 - E.R. Gauthier, Ph.D.

  29. DNA replication4) Proofreading CHMI 2227 - E.R. Gauthier, Ph.D.

  30. DNA sequencing CHMI 2227 - E.R. Gauthier, Ph.D.

  31. PCR CHMI 2227 - E.R. Gauthier, Ph.D.

  32. PCR CHMI 2227 - E.R. Gauthier, Ph.D.

  33. CHMI 2227 - E.R. Gauthier, Ph.D.

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