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Current and Former Staff of the Animal Resources Division, Health Canada 2001 Study James Brooks CIHR/CBS Post Doctoral

Simian Foamy Virus Infection Among Individuals Occupationally Exposed to Non Human Primates. Current and Former Staff of the Animal Resources Division, Health Canada 2001 Study James Brooks CIHR/CBS Post Doctoral Research Fellow

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Current and Former Staff of the Animal Resources Division, Health Canada 2001 Study James Brooks CIHR/CBS Post Doctoral

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  1. Simian Foamy Virus Infection Among Individuals Occupationally Exposed to Non Human Primates Current and Former Staff of the Animal Resources Division, Health Canada 2001 Study James Brooks CIHR/CBS Post Doctoral Research Fellow Erling Rud Head, Clinical Research, Animal Resources Division, HPFB Paul Sandstrom Associate Director Bureau of HIV/AIDS, STD & TB, CIDPC

  2. Rationale for the Health Canada SFV Study Trends in primate importation/utilization Description of Health Canada Animal Resources Division primate colony Results of Health Canada Study of Human SFV Infection Among Animal Workers Outline

  3. Macaque spp. have historically been among the most common NHP’s used in research Currently cynomolgus macaques (Macaca fascicularis) represent the vast majority of imported NHP’s Despite frequent occupational exposure there had not been a documented case of human SFV infection originating from macaques Background

  4. Last 2 years importation of NHP in U.S.A.(1999, N = 12,744; 2000, N = 12,061) # of non-human primates Source: Tom Damercus, Division of Quarantine, National Center for Infectious Disease, Centers for Disease Control and Prevention N = 12,744 N = 12,061

  5. Species of NHP used in Canada (1995 - 1999) N = 1373 N = 1,131 N = 2150 N = 1,621 N = 875

  6. To screen for human SFV infection of macaque origin using assays optimized for macaque SFV Study Objective

  7. 875 Cynomolgus macaques imported from the Philippines 1983-1984 Currently houses approximately 240 monkeys Previously housed up to 1200 monkeys Prior to 1983 a mixture of cynomolgus and rhesus macaques Herpes B free colony 80% seropositive for SFV Health Canada Primate Colony

  8. Average Monthly NHP Colony Population by Year Number of animals

  9. Anonymous, un-linked convenience sample of workers having contact with non-human primates or their blood and tissues Screened using immunoblot Target antigen SFV1, SFV3, SFV6 & SFV-HC infected cell culture lysates Confirmation using PCR amplification from PBLs of a 153 bp fragment located within the pol gene Study Design

  10. Occupations of Study Participants

  11. Average age 45 years M:F Ratio 56:44 Average of 13 years of exposure to Cynomolgus macaques Average of 10 years of exposure to Rhesus macaques Characteristics of Study Participants

  12. Person Years of Exposure to NHP Species

  13. Exposure Patterns

  14. Total Number of Bites Reported By Species

  15. 2 out of the 46 participants have positive serological tests for foamy virus infection (4% of study participants) 1 of the 46 participants has been confirmed as infected with SFV by PCR from PBLs Preliminary evidence suggests that this human SFV infection is of macaque origin Results of SFV Study

  16. Representative Screening Immunoblot Human 2 Chimp Baboon Cyno AGM Human 1 MW kDa 112 SFV HC/1/3/6 Infected Cell Lysates 81 50 36 Cf2Th/BHK21 Uninfected Host Cell Lysates 112 81 50 36

  17. Both individuals report : prolonged and ongoing exposure to cynomolgus macaques previous exposure to rhesus macaques events involving exposure to blood and/or body fluids, including bites, scratches or surgical injuries, from macaque species Exposure Histories of SFV Infected Humans

  18. 1000 2000 3000 4000 5000 6000 7000 8000 9000 10 000 11 000 12 000 13 000 0 SFV Genome Structure taf pol LTR LTR gag env Accessory Genes Location of 153 bp PCR Fragment in pol gene

  19. Neighbor-Joining Phylogenetic Analysis c1f 98 c3f Human 1 80 c10f 36 SFV 1 (macaque) 49 Hamlyn’s guenon African Green Monkey 0.05 46 SFV 3 90 Drill 57 Mandrill Patas Monkey 29 Papio urinus 26 Baboon 64 Papio anubis 92 93 Papio cynocephalus Gorilla Chimpanzee 99 HFV Courtesy of William Switzer, CDC

  20. 3% of eligible Canadians donate blood on a regular basis (Canadian Blood Services) 54% (25/46)of study participants reported at least one donation of blood or blood products ever. 88% (22/25) of Animal workers that reported blood donation also reported bites by NHP’s Pattern of Blood Donation

  21. Canadian Institutes of Health Research Canadian Blood Services Health Canada, CIDPC, PPHB Dr. Paul Gully Dr. Donald Sutherland Rick Pilon Chris Scherf Health Canada, HPFB Dr. Jocelyn Fournier University of Ottawa Dr Francisco Diaz-Mitoma Esoterix Inc. Dr. Richard Heberling Acknowledgements

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