Albumin Analysis by Potentiometric Method

1. Albumin Analysis by Potentiometric Method

2. Procedure

3. Cleaning of Al2O3 substrate Acetone ultrasonification for 5 mins Ethanol ultrasonification for 5 mins Drying in oven Deionizied water ultrasonification for 3 mins

4. Preparation of Au electrode Annealing Au thick film by programming Imprinting Au gel on substrate by halftone Drying Au electrode by N2 gas at room temperature Etching by 1.00 N NaOH and HCl solutions for 10 mins, respectively, then, washing by H2O thoroughly

5. Modification of Au electrode Modification of Au electrode in thiol solution 12 hrs at least under room temperature Preparation of 1.00 mM thiol ethanol/ water (4/1 v/v ) solution Washing electrode by deionizied water and ethanol thoroughly Drying modified electrode by N2 at room temperature

6. Preparation of Au MIP sensing film Photo-polymerization by 365 nm UV lightening for 36 hrs under N2 circumstance Spin-coating albumin containing reactive solution on Au electrode Washing out albumin molecule by 20 wt % methanol aqueous solution and detected by UV/Vis No albumin was detected in the eluted solution by the UV/Vis

7. Stabilization of electrode potential in open circuit Preparation of electrode Determine V of electrode after albumin addition Linear regression of V and albumin concn. Experimental Sequence of Potentiometric Method