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Isabel Borja NILE 2011

CLIL Project. Professional English for Clinical Microbiology Answer key Unit 2. Isabel Borja NILE 2011. Answer key Lesson 2.1 – Identification of Bacteria. Activity 1.a . Steps in identification:. Activity 1.b . Steps in identification:. Activity 2 .

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Isabel Borja NILE 2011

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  1. CLIL Project Professional English for Clinical Microbiology Answer key Unit 2 Isabel Borja NILE 2011

  2. Answer key Lesson 2.1 – Identification of Bacteria

  3. Activity 1.a Steps in identification:

  4. Activity 1.b Steps in identification:

  5. Activity 2 Preliminary identification: cultural and morphologic characteristics

  6. Activity 3.a Haemolysis  Text frame:

  7. Activity 3.a Haemolysis Identify in the image below the three kind of haemolysis: Alfa haemolysis Beta haemolysis Gamma haemolysis

  8. Activity 3.b Catalase test  Text frame:

  9. Activity 3.b • Catalase test •  Questions: • Complete the chemical reaction that involves catalase: • catalase • 2 H2O22 H2O + O2 • (ii) How shall we report the catalase test on the image and why. • Catalase positive. Because we may see bubbles produced by gaseous oxygen released in the chemical reaction.

  10. Activity 3.c Oxidase test  Text frame:

  11. Activity 3.c Oxidase test  Questions: Compare both images: Image 1 - Swab method: oxidase + Image 2 – Oxidase + (i) Deduct how the oxidase test in image 2 has been performed and the name of the method. Kovac´s reagent has been added directly to the plate. We may call this method: plate method. (ii) Do we need to take any precaution when using this second method? We cannot use the plate method with media that contain dyes because we may obtain a false positive because of the dye.

  12. Answer key Lesson 2.2 – Biochemical Tests

  13. Activity 1 Fundamentals:

  14. Activity 2.a

  15. Activity 2.a Tryptophanase Yellow Violet Kovac´s Indole reagent β-galactosidase

  16. Activity 2.b Taking biochemical tests to pieces

  17. Activity 3 COMMERCIAL KITS

  18. Answer key Lesson 2.3 – Variability of Bacteria

  19. Activity 1 LIMITS FOR VARIABILITY: 1.- When percentages are 98 or 99, that is over85% and it means that almost all the isolated cultures will give the same result and we may consider the species is positive to the test, but even so, we will find some strain will be negative. These tests have high significance because statistically they have quite sure results. 2.- On the other hand we have some percentages that are not so clear. Percentages around 85% may be considered as positive reactions of the species, but accepting this means that nearly 15% are negative strains. The one that has only 65% will be considered indeterminate for the referred test and that means that the test in this case has no significance. 3.- The most puzzling situation here is that of E. coli for Indol test: 50% means that halfthe isolated strains will be positive and halfnegative. The most indeterminate result we could ever have. For percentages under 50% we may apply the same reasoning as before for percentages about65%.

  20. Activity 2.a

  21. Activity 2.b Making it clear:

  22. Activity 3 Functional matrix:

  23. Answer key Lesson 2.4 – Susceptibility Tests

  24. Activity 1 Fundamentals of disc diffusion test: Resistant Susceptible

  25. Activity 2 Kirby-Bauer Method

  26. Activity 3.a Interpreting diameters:

  27. Activity 3.b Interpreting diameters:

  28. Activity 4 Using breakpoint tables:

  29. Activity 5 Using breakpoint tables: life test

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