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Lecture 5

Lecture 5. August 10, 2005. Lehninger Chapter 14 (4 th ed.) complete sections 14.2 and 14.3 skip section 14.4 for now. Lehninger Chapter 15 (4 th ed.). METABOLISM OF GLYCOGEN. Enzymes required for galactose metabolism. kinase. Galactose Galactose 1-P. UDP-Glu.

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Lecture 5

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  1. Lecture 5 August 10, 2005 Lehninger Chapter 14 (4th ed.) complete sections 14.2 and 14.3 skip section 14.4 for now Lehninger Chapter 15 (4th ed.) METABOLISM OF GLYCOGEN

  2. Enzymes required for galactose metabolism kinase Galactose Galactose 1-P UDP-Glu ATP ADP Uridyl transferase epimerase UDP-Gal Glucose 1-P phosphoglucomutase Glucose 6-P

  3. seen by electron microscopy Figure 7-14b

  4. 3 enzymes of glycogen breakdown(Glycogenolysis) • glycogen phosphorylase • glycogen debranching enzyme • phosphoglucomutase

  5. Non-reducing end Reducing end Figure 7-11

  6. Can reduce Cu2+ Oxidized sugar Cannot reduce Cu2+ Figure 7-10 Note this shows the β, not α configuration

  7. OH Figure 15-3

  8. Pyridoxal phosphate cofactor 4 3 2 1 4 3 2 1 Figure 15-4

  9. Pyridoxal phosphate cofactor Figure 15-3

  10. 3 enzymes of glycogen breakdown(Glycogenolysis) • glycogen phosphorylase • glycogen debranching enzyme [oligo (α1→6) to (α1→4) glucantransferase] • phosphoglucomutase

  11. Figure 7-15b

  12. 4 3 2 1 4 3 2 1 1 4 3 2 4 3 2 1 Figure 15-4

  13. Hydrolysis, NOT Phosphorolysis Figure 15-4

  14. 3 enzymes of glycogen breakdown(Glycogenolysis) • glycogen phosphorylase • glycogen debranching enzyme • Phosphoglucomutase Glu-1-P ↔ Glu-6-P

  15. Glu 1-P  Glu 6-P phosphoglucomutase Figure 15-5

  16. LNC 15.13

  17. GLYCOGEN METABOLISM GLYCOGEN SYNTHESIS LNC Chapter 15 (Section 1)

  18. UDP glucose is the activated precursor p. 565

  19. Glu + ATP → Glu-6-P + ADP Enzymes: hexokinase I and hexokinase II in muscle, hexokinase IV (glucokinase) in liver

  20. Glu-6-P ↔ Glu-1-P Phosphoglucomutase

  21. Glu-1-P + UTP → UDP-Glu + PPi Glu-1-P UDP-Glu Figure 15-7

  22. Start with glycogenin, a primer and enzyme. Muscle form and Liver form (glycogenin-2) + Figure 15-10 UDP-glucose

  23. Figure 15-11a

  24. LNC (3rd ed) 20-14 (not found in 4th edition)

  25. Figure 15-8

  26. Figure 15-9

  27. Figure 15-11b

  28. CONTROL OF GLYCOGEN SYNTHESIS AND BREAKDOWN

  29. Fig 15-13

  30. GLYCOGEN UDP-Glu Glu-1P Glu-6P Fru-6P Fru-1,6 bis P DHAP + GAP synthase phosphorylase Fructose-1,6-bis phosphatase Phosphofructokinase (PFK-1)

  31. Fig 15-14

  32. Covalent modifications Ligand-induced conformational changes

  33. Fig 15-24

  34. Fig 15-26

  35. adenyl cyclase ATP cAMP phosphodiesterase ADP AMP

  36. guanyl cyclase GTP cGMP phosphodiesterase

  37. LNC 12-15

  38. hormone Glycogen ==> Glu-1-P

  39. Fig 15-25

  40. Fig 15-27

  41. Fig 15-29

  42. Adenylate kinase 2 ADP ATP AMP + K = 0.44

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