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PCR condition

연대의대 소아과 - PCR results ( gDNA 2 ). PCR condition. EF taq Tm : 45~60 ℃ Template : gDNA 2 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃. 4 Kb.

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PCR condition

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  1. 연대의대 소아과 - PCR results (gDNA 2) PCR condition EF taq Tm : 45~60 ℃ Template : gDNA 2 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 4 Kb Tm: 45 / 47.5 / 51.4 / 53.9 / 56 / 58.8 (℃) <결과> · gDNA 2를 template로 하여 4kb size의 PCR product를 얻어 sequencing 의뢰함

  2. 연대의대 소아과 - PCR results (gDNA 1) PCR condition EF taq Tm : 45~56 ℃ Template : gDNA 1 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 4 Kb Tm: 51.6 / 53.1 / 54.3 / 55.2 (℃) <결과> · gDNA 1을 template로 하여 4kb size의 PCR product를 얻어 sequencing 의뢰함

  3. 연대의대 소아과 - PCR results gDNA 1 gDNA 2

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