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Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology

Recombinant mite allergens for diagnosis and immunotherapy in mite allergic patients. Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology Medical University of Vienna, Austria. Groups of mite allergens. Isolation and characterization

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Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology

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  1. Recombinant mite allergens for diagnosis and immunotherapy in mite allergic patients Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology Medical University of Vienna, Austria

  2. Groups of mite allergens

  3. Isolation and characterization of Der p allergens Baldo & Donovan, 1988

  4. Expression and purification of the clone 30-derived allergen

  5. Diagnosis of mite allergy • with mite extracts, which contain a mixture of allergenic • and non-allergenic components • extracts are standardized for Der p 1 and Der p 2 • some other important allergens are underrepresented • in mite extracts • - extracts do not allow a component-specific diagnosis Development of new diagnostic concepts with recombinant mite allergens

  6. IgE-reactivity profiles to Der p allergens

  7. Immunotherapy with mite extracts - No patient-tailored therapy is possible - sensitization to new allergens is possible - Induction of severe anaphylactic side effects is possible Recombinant mite allergens for immunotherapy Production of hypoallergenic derivatives of mite allergens with reduced allergenic activity

  8. IgG Abs induced with rDer p 2 derivatives inhibit patients‘ IgE binding to Der p 2 wildtype

  9. Summary • Identification of new mite allergens • - A combination of Der p 1 and Der p 2 allows • diagnosis of almost all mite allergic patients • Production of hypoallergenic derivatives of Der p 2 • with reduced allergenic activity and retained immunogenicity

  10. Wayne Thomas Telethon Institute of Child Health Research, Perth University of Western Australia Friedrich Horak Department of Otorhinolaryngology Medical University of Vienna Group Immunotechnology Margit Weghofer Kuan-Wei Chen Anne Casset Peter Valent Division of Hematology and Hemostaseology Medical University of Vienna Walter Keller Institute of Chemistry Karl-Franzens University of Graz Gabrielle Pauli Hôpitaux Universitaires de Strasbourg Medical University of Vienna, Department of Pathophysiology, Division of Immunopathology Rudolf Valenta Adriano Mari Allergy Unit National Health Service, Rome Marianne van Hage Karolinska Institutet, Stockholm This work was supported by grants F1803, F1805, F1809 and F1815 of the Austrian Science Fund.

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