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Gene Query

Gene Query. http://www.ncbi.nlm.nih.gov/. HBB gene for homo sapiens. Our query will give the following results. Sequences available. Proteins available. Expression data, available from several high-throughput transcriptome technologies. Micorarray Experiment. Sequences available.

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Gene Query

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  1. Gene Query http://www.ncbi.nlm.nih.gov/ HBB gene for homo sapiens

  2. Our query will give the following results Sequences available Proteins available Expression data, available from several high-throughput transcriptome technologies. Micorarray Experiment

  3. Sequences available We check for sequences

  4. Info concerning the GENE Info concerning the HBB Nucleotide Sequence

  5. Info concerning the GENE Info concerning the HBB Nucleotide Sequence Lets check the nucleotide sequence by opening the link

  6. Info for the sequence Accession from RefSeq DB Latest annotation Length “vesrion” and “gi” accessions: CHECK LINK FOR INFO: http://www.ncbi.nlm.nih.gov/Sitemap/sequenceIDs.html

  7. Lower part of the previous file Additional links

  8. More choices of visualizing the sequence and the related information

  9. More choices of visualizing the sequence and the related information Lets choose the coding region (CDS). This will give the minimum information concerning Gene and CDS (see next slide)

  10. Start end of GENE Start end of coding region Link to other databases

  11. Lets extract the coding sequence by clicking at the CDS link

  12. It opens the part concerning the cds Check the different (lower) length to that of the gene Upper part of the file Lower part of the file terminates with the coding sequence HOW TO EXTRACT

  13. Above we have the option to choose the format. Since we want to extract the Coding sequence, we choose FASTA format.

  14. WE SAVE THE NUCLEOTIDE SEQUENCE OF HBB. WE MAY NOW OPEN AND VISUALIZE THE SAVED SEQUENCE USING A TEXT EDITOR

  15. LETS GO BACK TO THE INITIAL QUERY Info concerning the GENE Info concerning the HBB Nucleotide Sequence Lets check the GENE INFORMATION

  16. SEVERAL FEATURES FOR THE GENE UNDER EXAM

  17. GENE STRUCTURE Plus Genomic info

  18. Info for the 5’ and 3’ UTR, introns and exons. Information for position on GENOME

  19. GENOME VIEWER

  20. OUR GENE

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