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Prepare a Wet Mount

Prepare a Wet Mount. CAPT Mike Hughey, MC, USNR. First, a Video. Obtain a Specimen. Spatula Cotton-tipped Applicator Obtain directly or from inside curve of speculum. Place on Microscope Slide. Use very small amount Too much makes the slide too thick and unreadable. Add NaCl and KOH.

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Prepare a Wet Mount

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  1. Prepare a Wet Mount CAPT Mike Hughey, MC, USNR

  2. First, a Video

  3. Obtain a Specimen • Spatula • Cotton-tipped Applicator • Obtain directly or from inside curve of speculum

  4. Place on Microscope Slide • Use very small amount • Too much makes the slide too thick and unreadable

  5. Add NaCl and KOH • One drop • Mix well • Two slides vs. one slide?

  6. Add Coverslip • Remove excess fluid so the coverslip is stable. • Heat the KOH slide?

  7. Microscopic Examination • Start with low power • Rarely need to go above medium power • Focus up and down through the plane of the cells

  8. Yeast Single branch Tangle of threads

  9. Watch this video

  10. Trichomonas • About the same size as a white blood cell • Smaller than a vaginal epithelial cell • Violent motion

  11. Watch this video

  12. Bacterial Vaginosis • Clue cells • Vaginal epithelial cells studded with bacteria • Smudged, granular appearance • Indistinct borders Clue Cell Normal Vaginal Epithelial Cell

  13. Clue Cells

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