1 / 14

Advances in DNA technology (Post Watson and Crick) DNA profiling Genomic sequencing

Advances in DNA technology (Post Watson and Crick) DNA profiling Genomic sequencing Genetic engineering Gene therapy . Techniques involved Cutting DNA Separating pieces of DNA according to their size Copying DNA Sticking pieces of DNA together DNA probes .

fabian
Download Presentation

Advances in DNA technology (Post Watson and Crick) DNA profiling Genomic sequencing

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Advances in DNA • technology • (Post Watson and Crick) • DNA profiling • Genomic sequencing • Genetic engineering • Gene therapy

  2. Techniques involved • Cutting DNA • Separating pieces of DNA according to their size • Copying DNA • Sticking pieces of DNA together • DNA probes

  3. How do you sequence a genome? First – Map the genome The shear it The place the fragments into BAC (bacterial artificial chromosomes) then put the BACs into bacteria Culture the bacteria until you have many of them Harvest the DNA then separate out the different bits of DNA using gel electrophoresis Sequence the bits of DNA The sequences overlap, so use a computer program to work out the order they go in

  4. A G G C G A T T A G C A G C T TT G C A C T C A C T A T TT G G A T C G A T C A T G C C A T G G

  5. Questions…. 1. A particular gene is found in all small pox viruses, but not in cow pox viruses. What would you conclude about this gene? 2. A particular gene is found, in a very similar form, in almost all living organisms, what would you conclude about this gene. 3. Suggest an example of a gene that might be found in all plants, but not animals.

  6. Gel electrophoresis Used to separate out pieces of DNA on the basis of their size

  7. Gel electrophoresis Used to separate out pieces of DNA on the basis of their size

  8. Gel electrophoresis Used to separate out pieces of DNA on the basis of their size + -

  9. Gel electrophoresis Used to separate out pieces of DNA on the basis of their size + -

  10. Gel electrophoresis Used to separate out pieces of DNA on the basis of their size + -

  11. http://plantandsoil.unl.edu/croptechnology2005/gen/animationOut.cgi?anim_name=Gel_electrophoresis.swfhttp://plantandsoil.unl.edu/croptechnology2005/gen/animationOut.cgi?anim_name=Gel_electrophoresis.swf Gel electrophoresis Used to separate out pieces of DNA on the basis of their size + -

More Related