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Stres Oksidatif pada Tumbuhan

Stres Oksidatif pada Tumbuhan. ABA dan Etilen Memicu Pembentukan ROS. Rinaldi Rizal Putra 12/338166/PBI/01056 Darussalam 12/339652/PBI/01072. Program Studi Biologi Program Pascasarjana Fakultas Biologi Universitas Gadjah Mada 2013. Pendahuluan.

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Stres Oksidatif pada Tumbuhan

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  1. Stres Oksidatif pada Tumbuhan ABA dan Etilen Memicu Pembentukan ROS Rinaldi Rizal Putra 12/338166/PBI/01056 Darussalam 12/339652/PBI/01072 Program Studi Biologi Program Pascasarjana Fakultas Biologi Universitas Gadjah Mada 2013

  2. Pendahuluan • Lingkungan  memiliki faktor-faktor yang dapat berubah secara drastis. • Perubahan tersebut dapat mendatangkan efek yang merugikan bagi kesintasan, pertumbuhan, dan reproduksi tumbuhan. • Tumbuhan tidak dapat menolerensi stres lingkungan tersebut. Biokimia Adaptasi - UGM - 2013

  3. lanjutan... Biokimia Adaptasi - UGM - 2013

  4. Stres Oksidatif? Biokimia Adaptasi - UGM - 2013

  5. Penyebab Stres Oksidatif POLLUTANT HERBICIDES Oxidative stress PHOTOSENSITIZING TOXIN METAL DROUGHT Biokimia Adaptasi - UGM - 2013

  6. Equilibrium between AOX & ROS Biokimia Adaptasi - UGM - 2013 (Gill and Tuteja, 2010)

  7. Biokimia Adaptasi - UGM - 2013

  8. Reactive Oxygen Species (ROS) Non-Radicals: H2O2Hydrogen peroxide HOCl- Hypochlorous acid O3Ozone 1O2 Singlet oxygen ONOO- Peroxynitrite Radicals: O2.- Superoxide OH. Hydroxyl RO2. Peroxyl RO. Alkoxyl HO2.Hydroperoxyl Reactive Nitrogen Species (RNS) Non-Radicals: ONOO-Peroxynitrite ROONO Alkyl peroxynitrites N2O3 Dinitrogen trioxide N2O4 Dinitrogen tetroxide HNO2 Nitrous acid NO2+Nitronium anion NO- Nitroxyl anion NO+ Nitrosyl cation NO2Cl Nitryl chloride Radicals: NO. Nitric Oxide NO2. Nitrogen dioxide Biokimia Adaptasi - UGM - 2013

  9. Biological Pathways for Oxygen Reduction From: McMurry and Castellion “Fundamentals of general, organic and biological chemistry” Biokimia Adaptasi - UGM - 2013

  10. Sumber ROS pada Sel Tumbuhan Biokimia Adaptasi - UGM - 2013

  11. Biokimia Adaptasi - UGM - 2013

  12. NADPH oxidase as a source of ROS Present mainly in neutrophils (oxidative burst), but also in many other cell types ANTIOXIDANTS & REDOX SIGNALING Volume 8, Numbers 3 & 4, 2006 Activation of the gp91phox (NOX2) containing NOX complex of phagocytes involves phosphorylation of the cytoplasmic regulator p47phox, with the translocation of the cytoplasmic p47phox, p67phox, and p40phox regulatory components to the plasma membrane to interact with flavocytochrome-b558, which is composed of gp91phox and p22phox. Activation of the complex also involves guanine nucleotide exchange on the GTP-binding protein RAC stimulated by guanine nucleotide exchange factors. Guanine nucleotide exchange on RAC is associated with release of RhoGDI and translocation of RAC from the cytosol to the NOX complex at the plasma membrane. Biokimia Adaptasi - UGM - 2013

  13. Reaksi-reaksi yang menghasilkan hidrogen peroxide antara lain 1. 2O2+ 2Fdred2O2-+ 2Fdox 2. 2O2-+ 2H+H2O2+ O2 3. 2O2- + 2H+H2O2 + O2 • Hydroxyl radical (OH) SOD

  14. Pembentukan dan saling tukar ROS yang dibentuk dari oksigen singlet. Biokimia Adaptasi - UGM - 2013

  15. Sumber dan lokasi pembentukan ROS pada sel tumbuhan Biokimia Adaptasi - UGM - 2013

  16. Exogenous sources of free radicals • Radiation • UV light, x-rays, gamma rays • Chemicals that react to form peroxides • Ozone and singlet oxygen • Chemicals that promote superoxide formation • Quinones, nitroaromatics, bipyrimidiulium herbicides • Chemicals that are metabolized to radicals • e.g., polyhalogenated alkanes, phenols, aminophenols • Chemicals that release iron • ferritin Biokimia Adaptasi - UGM - 2013

  17. Pembentukan ROS yang dipicu oleh ABA dan Etilen Biokimia Adaptasi - UGM - 2013

  18. Stres Oksidatif oleh ABA Biokimia Adaptasi - UGM - 2013

  19. Biosintesis Etilen pada Tanaman Biokimia Adaptasi - UGM - 2013

  20. Stres Oksidatif oleh Etilen Eksogen Biokimia Adaptasi - UGM - 2013

  21. Efek Stres Oksidatif Etilen Eks Biokimia Adaptasi - UGM - 2013

  22. Laju respirasi pada mentimun disimpan pada 130C kontrol dan perlakuan dengan etilen 10,100,500, 1000 µLL-1 Biokimia Adaptasi - UGM - 2013

  23. Perubahan warna pada permukaan mentimun A. mentimun disimpan pada 130C kontrol dan perlakuan dengan etilen 10,100,500, 1000 µLL-1 dan B. Disimpan pada udara ambien (kontrol) dan perlakuan dengan etilen 10 µL selama 12 jam,2, 4 hari dan terus menerus. Biokimia Adaptasi - UGM - 2013

  24. A. Pelunakan buah mentimun disimpan pada 130C kontrol dan perlakuan dengan etilen 10,100,500, 1000 µLL-1 B. Pelunakan mesokarpium buah mentimun udara (kontrol) dan perlakuan etilen 10 µLL-1 selama 12 jam, 2 hari, 4 hari. Biokimia Adaptasi - UGM - 2013

  25. Pembentukan ROS pada buah mentimun disimpan pada 130C kontrol dan perlakuan terus menerus dengan etilen 10 µLL-1. Biokimia Adaptasi - UGM - 2013

  26. Simpulan 1 2 3 Biokimia Adaptasi - UGM - 2013

  27. lanjutan... 4 5 Biokimia Adaptasi - UGM - 2013

  28. Sekian dan terima kasih..... Rinaldi Rizal Putra 12/338166/PBI/01056 Darussalam 12/339652/PBI/01072 Biokimia Adaptasi - UGM - 2013

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