1 / 7

Bispecific Antibody Overview

Bispecific Antibody (BsAb) are antibodies that can simultaneously bind two separate and unique antigens (or different epitopes of the same antigen). The primary application of BsAb has been to redirect cytotoxic immune effector cells for the enhanced killing of tumor cells by antibody-dependent cell-mediated cytotoxicity (ADCC) and other cytotoxic mechanisms mediated by the effector cells.

genscriptprobio
Download Presentation

Bispecific Antibody Overview

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Bispecific Antibody Overview Bispecific Antibody (BsAb) are antibodies that can simultaneously bind two separate and unique antigens (or different epitopes of the same antigen). The primary application of BsAb has been to redirect cytotoxic immune effector cells for the enhanced killing of tumor cells by antibody-dependent cell-mediated cytotoxicity (ADCC) and other cytotoxic mechanisms mediated by the effector cells.

  2. Bispecific Antibody Structure and Formats

  3. Schematic of IgG Antibody Production Using Transient Expression

  4. IgGAntibody Production Steps • IgG Antibody Gene Synthesis and Subcloning: After customer's approval, target DNA sequences are designed and synthesized. The complete sequences are subcloned into a proprietary vector for expression. • IgG Antibody Cell Culture and Transient Transfection: Cells are grown in serum-free media. Recombinant plasmids encoding H and L chains are transiently co-transfected to suspension cell cultures. Cell culture supernatant is collected on day-5, post-transfection for SDS-PAGE analysis, in order to evaluate expression levels. • IgG Antibody Purification and Analysis: Purification is carried out using protein A column. Eluted fractions are analyzed by SDS-PAGE and Western blot and pooled and buffer exchanged to the final storage buffer. • Deliverables: Synthetic genes in pUC57 vector, optimized gene sequence report and purified antibody at listed amount and purity were delivered to the client along with QC data and comprehensive report.

  5. Bispecific IgG Production Purified Bispecific IgG Antibody SDS-PAGE (left) and Western Blot of the same panel (right) under reducing and non-reducing conditions

  6. We Also Provide Services Like • plasmid manufacturing • biologics CMC • PK kit • single B cell sorting • antibodies characterization • lentiviral vector production • LVV production • AAV packaging • GMP lentivirus • CAR-T plasmid • biologics CDMO • cell line development • Biologics manufacturing • Preclinical CMC • single domain antibody • ADA kit • antibody humanization • hybridoma sequencing

  7. Thanks For Watching www.genscriptprobio.com

More Related