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Using Real-Time PCR to Determine Blood Type

Using Real-Time PCR to Determine Blood Type. Lightcycler data. Example: Blood Type SNPs. Use two assays in a lightcycler: B assay (which detects the SNP at 796) O assay (which detects the SNP at 261). Blood Typing. B Assay. O Assay. "O" (deletion). "not-O" (G). " "not-B" (C). "B"

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Using Real-Time PCR to Determine Blood Type

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  1. Using Real-Time PCR to Determine Blood Type

  2. Lightcycler data

  3. Example: Blood Type SNPs • Use two assays in a lightcycler: • B assay (which detects the SNP at 796) • O assay (which detects the SNP at 261).

  4. Blood Typing B Assay O Assay "O" (deletion) "not-O" (G) ""not-B" (C) "B" (A) fluorescence fluorescence 55 65 55 62 temp (C) temp (C)

  5. Group Practice: What’s this person’s blood type? B Assay O assay "O" (deletion) "not-O" (G) ""not-B" (C) "B" (A) fluorescence fluorescence Type BO 55 65 55 62

  6. What is the blood type? "O" (deletion) "not-O" (G) ""not-B" (C) "B" (A) fluorescence fluorescence Type AO 55 65 55 62

  7. What is the blood type? "O" (deletion) "not-O" (G) ""not-B" (C) "B" (A) fluorescence fluorescence Type OO 55 65 55 62

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