1 / 15

Introduction to the NGC Chromatographic System

Introduction to the NGC Chromatographic System. Protein purification systems – yesterday & today. Simplified, a chromatography system is just : Pumps Valves (Columns) Detector(s) Fraction Collector. NGC System Capabilities. Bulletins 6286, 6326, and 6608.

gpfister
Download Presentation

Introduction to the NGC Chromatographic System

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Introduction to the NGC Chromatographic System

  2. Protein purification systems – yesterday & today • Simplified, a chromatography system is just: • Pumps • Valves • (Columns) • Detector(s) • Fraction Collector

  3. NGC System Capabilities Bulletins 6286, 6326, and 6608

  4. A chromatography platform that aligns to your needs Quest™ & Quest™ Plus Workhorse system for the purification of proteins peptides and nucleic acids Scout™ & Scout™ Plus All purpose purification system with buffer scouting Versatile automated purfication system for advanced method development Discover ™

  5. Expandable system that grows with you Get what you need today with a single, scalable platform that grows and adapts to your throughput and purification needs over time Tier Expansion

  6. Plug-n-Play Modular Design • A truly modular design • Plug-n-play modules can be easily moved within the system • New modules can be added to expand system capabilities as your purification needs change Plug-n-Play Modules Note system must be switched off before modules are removed

  7. ChromLab Software Highlights • Touchscreen • Intuitive • Method Templates • Column Library • Scouting • Multi-D • Import GE Unicorn SW and Bio-Rad DuoFlow files • Analysis made easy • One click peak integration • Easy overlay • Fraction heat map • Concentration determination • User Management Edition and Security Edition • FREE Bulletin 6675

  8. The Heart of the System - Pumps • Two versions available, 10 and 100 mL/min • Buffer blending module allows for double flow and simplified buffer preparation

  9. Inlets – Great for Automation • A buffer inlet valve provides automated buffer switching.

  10. Sample Loading – Volume is Key • Volumes >10 ml use a sample loading pump with air sensor. • Smaller volumes should be done with loops.

  11. Column Switching Valve • Allows reverse flow • CSV can also serve as a “loop multiplier” allowing for up to 5 sample loops

  12. Detectors – The “eyes” of the system • Single wavelength for labs doing simple protein OR DNA purification • Multi-Wavelength is great for labs that: • Purify Protein/DNA complexes • Purify a fluorescently labeled protein • Purify a protein with no signal at 280nm Human Eyes Superman’s Eyes

  13. Outlet – Large Volume Fractionation • Useful for labs doing large volume work • Able to save the “flow through” in one large container rather using the BioFrac

  14. NGCTM system capabilities Single-WL Detector • 255/280nm BioFracTM Fraction Collector • Unlimited Options • 180 x 12mm tubes • 168 x 1.5ml tubes • 120 x 15mm tubes • 24 x 30mm tubes • 4 x 96, 48-, 24- or 12-position micro-plates • 20 x unlimited vol. adaptor • Outlet valve System Pump • 10 ml/min, 25.2 MPa • 100 ml/min, 10 MPa Column Valve • Up to 5 col. • Int. Bypass • Reverse Flow • Pre- & Delta Col. Pressure Sample Inj. Valve • No re-plumbing Conductivity Monitor • 0.01 – 999 mS/cm Sample Pump • Ideal for Affinity • Integrated • 100 ml/min, 10 MPa • Int. Press. Sensor Buffer Inlet Valve (A/B) • Up to 8 Inlets each Multi-WL Detector • 190-800nm (UV/Vis) • Up to 4 simultaneous • 2, 5 & 10 mm Path lengths Buffer Blending Valve • pH Scouting • Doubles pump flow with gradient Air Sensor • Protects Column • Up to 8 Inlets (7 Buffers, 1 Sample) pH Probe & Valve • In situ calibration • Integrated bypass

  15. Standout features • Point-to-Plumb™ • Pay for only what you need • Infinitely configurable • Tier expansion • Columns mount anywhere • Modules can go anywhere • Free software—for life! • Ten-second module swapping • Highly intuitive software

More Related