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Aerobic Gram-Positive Bacilli Part II Genital Culture Unit

Please click audio icon to hear Carol’s narration. Aerobic Gram-Positive Bacilli Part II Genital Culture Unit. Division of Medical Technology Carol Larson MSEd, MT(ASCP). Click icon for audio. Differentiation of Major GPR Genera. Gram stain Morphology Arrangement Formation of spores

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Aerobic Gram-Positive Bacilli Part II Genital Culture Unit

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  1. Please click audio icon to hear Carol’s narration Aerobic Gram-Positive BacilliPart IIGenital Culture Unit Division of Medical Technology Carol Larson MSEd, MT(ASCP)

  2. Click icon for audio Differentiation ofMajor GPR Genera • Gram stain • Morphology • Arrangement • Formation of spores • Catalase reaction

  3. Click icon for audio GPR Discussed in Student Lab • Spore-forming GPR, catalase positive • Bacillus species • Bacillus anthracis • Bacillus cereus • Nonspore-forming GPR, catalase positive • Listeria monocytogenes • Corynebacterium species (diphtheroids) • Corynebacterium diphtheriae • Corynebacterium jekeium

  4. Click icon for audio GPR Discussed in This Lecture • Nonspore-forming GPR, catalase negative • Gardnerella species • Erysipelothrix species • Lactobacillus species • Branching Nocardioform GPR • Nocardia species • Streptomyces species

  5. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Gardnerella vaginalis Gram Stain • Pleomorphic gram-variable bacilli • Non-sporulating • Specimen gram stain: • Clue cells

  6. Does not grow on sheep blood agar Human Blood Agar (V-agar, HBT-agar) Small colonies with diffuse zone of beta-hemolysis GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Gardnerella vaginalisColony Morphology

  7. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Gardnerella vaginalisIdentification • Catalase negative • Oxidase negative • Sodium hippurate usually positive • SPS sensitive

  8. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Gardnerella vaginalisClinical Significance • Normal vaginal flora • Bacterial vaginosis • Polymicrobic infection with Mobiluncus and Bacteroides • Diagnosis • Homogeneous, gray discharge • Clue cells • Amine or fishy odor when add 10% KOH • Culture not necessary for diagnosis

  9. What are the key identification characteristics to determine if Gardnerella vaginalis is the cause of bacterial vaginosis? Examine vaginal specimen for presence of a homogeneous, gray discharge, clue cells on the Gram stain, and an amine or fishy odor when adding 10% KOH. A culture is not recommended, but if done, G. vaginalis grows on V-agar/HBT-agar and it beta hemolytic.

  10. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Lactobacillus speciesGram Stain • Two types • Long slender GPR in chains • Short GPCB • Non-sporulating

  11. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Lactobacillus speciesColony Morphology • Grows on BAP • Multiple colony morphologies • Nonhemolytic or alpha hemolytic • Microaerophilic

  12. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Lactobacillus speciesIdentification • Catalase negative • Sucrose “F” • Vancomycin “R”

  13. GPR, pleomorphic, Non-spore forming, Catalase negative Click icon for audio Lactobacillus speciesClinical Significance • Normal flora • Mouth • GI tract • Female vaginal tract • Rarely pathogenic • Endocarditis • Meningitis

  14. What is the clinical significance of Lactobacillus species? It is considered normal flora of the mouth, gastrointestinal tract, and female genital tract

  15. Click icon for audio Review • Nonspore-forming GPR, catalase negative • Gardnerella species • Erysipelothrix species • Lactobacillus species

  16. Click icon for audio Aerobic GPRSummary • Nonspore-forming GPR, catalase negative • Gardnerella species • Erysipelothrix species • Lactobacillus species • Branching Nocardioform GPR • Nocardia species • Streptomyces species

  17. Who am I? HBT media shows beta-hemolysis Gram Stain Causes Bacterial Vaginitis Gardnerella vaginalis

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